Effect of the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) upon membrane ionic exchanges in sea urchin eggs

Journal Article · Fri Dec 01 04:00:00 EST 1989 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(89)90311-X· OSTI ID:5622905

Ciapa, B; Payan, P ^[1]; Allemand, D ^[2]

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1. UA 651 Centre National de la Recherche Scientifique, Nice (France)
2. Centre Scientifique de Monaco, Monte Carlo (Monaco)

The effect of TPA (12-O-tetradecanoylphorbol-13-acetate) upon ionic exchanges was investigated in eggs of the sea urchin Arbacia lixula. Ouabain-sensitive {sup 86}Rb uptake and amiloride-sensitive {sup 24}Na influx were dramatically stimulated after TPA addition, indicating an enhancement of total ionic permeabilities. Stimulation by TPA of both Na{sup +}/H{sup +} and Na{sup +}/K{sup +} exchanges was canceled by amiloride, suggesting that activation of protein kinase C elicits, via Na{sup +}/H{sup +} activity, stimulation of the sodium pump. However, TPA did not stimulate sodium pump activity and Na{sup +}/H{sup +} exchange at the same rate as fertilization, probably because of an absence of calcium-dependent events. Further fertilization of TPA pretreated eggs triggered an enhancement of sodium pump activity when the TPA treatment duration did not exceed 10 minutes. It is suggested that TPA activates preexisting transporting mechanisms in plasma membranes of unfertilized eggs (Na{sup +} stat, pH stat).

• OSTI ID: 5622905
• Journal Information: Experimental Cell Research; (United States), Journal Name: Experimental Cell Research; (United States) Vol. 185:2; ISSN ECREA; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALI META
ALKALI METAL COMPOUNDS
ALKALINE EARTH METAL COMPOUNDS
ANIMALS
AQUATIC ORGANISMS
BARYONS
BENTHOS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
CALCIUM COMPOUNDS
CARCINOGENS
CELL CONSTITUENTS
CELL MEMBRANES
DAYS LIVING RADIOISOTOPES
ECHINODERMS
EGGS
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTARY PARTICLES
ESTERS
FERMIONS
HADRONS
HOURS LIVING RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
INVERTEBRATES
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LIGHT NUCLEI
MEMBRANE TRANSPORT
MEMBRANES
MILLISEC LIVING RADIOISOTOPES
MINUTES LIVING RADIOISOTOPES
NUCLEI
NUCLEONS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PH VALUE
PHORBOL ESTERS
PROTONS
RADIOISOTOPES
RUBIDIUM 86
RUBIDIUM ISOTOP
SEA URCHINS
SECONDS LIVING RADIOISOTOPES
SODIUM 24
SODIUM COMPOUNDS
SODIUM ISOTOPES