Cutinase of Fusarium solani F. sp. pisi: mechanism of induction and relatedness to other Fusarium species
Thesis/Dissertation
·
OSTI ID:5622280
Three studies were made on the extracellular cutinase of the phytopathogenic fungus Fusarium solani f. sp. pisi. I. The production of cutinase was found to be induced in spores of F. solani f. sp. pisi, strain T-8, by cutin and cutin hydrolysate. Fractionation and analysis of the cutin hydrolysate indicated that dihydroxy-C/sub 16/ acid and trihydroxy-C/sub 18/ acid were the cutin monomers most active for inducing cutinase. Measurement of cutinase-specific RNA levels by dot-blot hybridization with a (/sup 32/P)-labeled cutinase cDNA showed that the cutinase gene transcripts could be detected within 15 min after addition of the inducers. The results indicated that the fungal spores have the capacity to recognize the unique monomer components of the plant cuticle and rapidly respond by the synthesis of cutinase. II. Analysis of the genomic DNA's of seven strains of F. solani f. sp. pisi indicated that both high and low cutinase-producing strains contain at least one copy of the cutinase structural gene and a homologous promoter region. The data suggest a different promoter sequence exists in these additional copies. III. Relatedness of five phytopathogenic Fusarium species to F. solani f. sp. pisi was determined by their cutinase antigenic properties and gene homologies of cutinase cDNA from F. solani f. sp. pisi. The results suggest that formae specialis of F. solani are phylogenetically identical and that F. solani is quite distinct from the other Fusarium species tested.
- Research Organization:
- Washington State Univ., Pullman (USA)
- OSTI ID:
- 5622280
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOLOGICAL EVOLUTION
BIOLOGY
CARBOHYDRATES
CHEMICAL ACTIVATION
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DNA
ENZYMES
FUNGI
FUSARIUM
GENETICS
HYDROLASES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLANTS
POLYSACCHARIDES
RADIOISOTOPES
RECOMBINANT DNA
RNA
SACCHARIDES
TRACER TECHNIQUES
59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOLOGICAL EVOLUTION
BIOLOGY
CARBOHYDRATES
CHEMICAL ACTIVATION
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DNA
ENZYMES
FUNGI
FUSARIUM
GENETICS
HYDROLASES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLANTS
POLYSACCHARIDES
RADIOISOTOPES
RECOMBINANT DNA
RNA
SACCHARIDES
TRACER TECHNIQUES