Assay of cysteine dioxygenase activity
Conference
·
· FASEB Journal (Federation of American Societies for Experimental Biology); (United States)
OSTI ID:5618638
- Cornell Univ., Ithaca, NY (United States)
It has been proposed that rat liver contains two cysteine dioxygenase enzymes which convert cysteine to cysteinesulfinic acid, one which is stimulated by NAD{sup +} and has a pH optimum of 6.8 and one which is not stimulated by NAD{sup +} and has a pH optimum of 9.0. This led the authors to reinvestigate assay conditions for measuring cysteine dioxygenase activity in rat liver homogenate. An HPLC method, using an anion exchange column (Dionex Amino-Pac{trademark} PA1 (4x250 mm)) was used to separate the ({sup 35}S)cysteinesulfinic acid produced from ({sup 35}S)cysteine in the incubation mixture. They demonstrated that inclusion of hydroxylamine prevented further metabolism of cysteinesulfinic acid. which occurred rapidly in the absence of hydroxylamine.
- OSTI ID:
- 5618638
- Report Number(s):
- CONF-9004153--
- Conference Information:
- Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 4:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
CARBOXYLIC ACIDS
CYSTEINE
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ENZYME ACTIVITY
ENZYMES
EVEN-ODD NUCLEI
GLANDS
HOMOGENATES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
LIVER
MAMMALS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
OXIDOREDUCTASES
OXYGENASES
PROTEINS
RADIOISOTOPES
RATS
REACTION KINETICS
RODENTS
SULFUR 35
SULFUR ISOTOPES
THIOLS
TRACER TECHNIQUES
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
CARBOXYLIC ACIDS
CYSTEINE
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ENZYME ACTIVITY
ENZYMES
EVEN-ODD NUCLEI
GLANDS
HOMOGENATES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
LIVER
MAMMALS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
OXIDOREDUCTASES
OXYGENASES
PROTEINS
RADIOISOTOPES
RATS
REACTION KINETICS
RODENTS
SULFUR 35
SULFUR ISOTOPES
THIOLS
TRACER TECHNIQUES
VERTEBRATES