Stromal cells in long-term murine bone marrow culture: FACS studies and origin of stromal cells in radiation chimeras

Journal Article · Thu May 01 04:00:00 EDT 1986 · Exp. Hematol.; (United States)

OSTI ID:5617705

Lennon, J E; Micklem, H S

Adherent layers from hematopoietically active long-term bone marrow cultures (LTBMC), incubated with fluorescent beads, were analyzed for autofluorescence and phagocytic ability, using a fluorescence-activated cell sorter (FACS). Four groups of cells were separated from the adherent layers, including a group of large polygonal fibroblastoid stromal cells. Long-term chimeras were made by lethal irradiation of CBA/Ca (CBA) and C57Bl6/J (B6) mice and repopulation with phosphoglycerate kinase (PGK-1) alloenzyme-congenic bone marrow cells. Hematopoietically active LTBMC were established from such chimeras, and donor and host contributions of FACS-sorted adherent-layer cells were measured. While macrophages and other hematopoietic cells were of donor origin, the fibroblastoid stromal cells were mainly or entirely host derived.

• Research Organization: Univ. of Edinburgh, England
• OSTI ID: 5617705
• Journal Information: Exp. Hematol.; (United States), Journal Name: Exp. Hematol.; (United States) Vol. 4; ISSN EXHMA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADHESION
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BODY
BONE MARROW
CELL CULTURES
CHIMERAS
CONNECTIVE TISSUE CELLS
FIBROBLASTS
FLUORESCENCE
HEMATOPOIETIC SYSTEM
LUMINESCENCE
MAMMALS
MICE
MOSAICISM
ORGANS
PHAGOCYTOSIS
RADIATION CHIMERAS
RODENTS
SOMATIC CELLS
TISSUES
VERTEBRATES