Stromal cells in long-term murine bone marrow culture: FACS studies and origin of stromal cells in radiation chimeras
Adherent layers from hematopoietically active long-term bone marrow cultures (LTBMC), incubated with fluorescent beads, were analyzed for autofluorescence and phagocytic ability, using a fluorescence-activated cell sorter (FACS). Four groups of cells were separated from the adherent layers, including a group of large polygonal fibroblastoid stromal cells. Long-term chimeras were made by lethal irradiation of CBA/Ca (CBA) and C57Bl6/J (B6) mice and repopulation with phosphoglycerate kinase (PGK-1) alloenzyme-congenic bone marrow cells. Hematopoietically active LTBMC were established from such chimeras, and donor and host contributions of FACS-sorted adherent-layer cells were measured. While macrophages and other hematopoietic cells were of donor origin, the fibroblastoid stromal cells were mainly or entirely host derived.
- Research Organization:
- Univ. of Edinburgh, England
- OSTI ID:
- 5617705
- Journal Information:
- Exp. Hematol.; (United States), Vol. 4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
FIBROBLASTS
PHAGOCYTOSIS
ADHESION
BONE MARROW
CELL CULTURES
FLUORESCENCE
MICE
RADIATION CHIMERAS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BODY
CHIMERAS
CONNECTIVE TISSUE CELLS
HEMATOPOIETIC SYSTEM
LUMINESCENCE
MAMMALS
MOSAICISM
ORGANS
RODENTS
SOMATIC CELLS
TISSUES
VERTEBRATES
560152* - Radiation Effects on Animals- Animals