Skin allografts in lethally irradiated animals repopulated with syngeneic hemopoietic cells
Thesis/Dissertation
·
OSTI ID:5617596
Total body irradiation and repopulation with syngeneic hemopoietic cells can be used to induce tolerance to major histocompatibility complex (MHC) mismatched heart and kidney grafts in rats and mice. However, this protocol does not work for MHC mismatched skin grafts in rats or mice. Furthermore, LEW rats that accept WF cardiac allografts after irradiation and repopulation reject subsequent WF skin grafts. Treatment of skin allograft donors with methotrexate prior to grafting onto irradiated and reconstituted mice resulted in doubling of the mean survival time. Analysis of which antigens provoked skin graft rejection by irradiation and reconstituted animals revealed the importance of I region antigens. Cardiac allograft acceptance by irradiated and reconstituted animals is mediated by suppressor cells found in the spleen. Adoptively tolerant LEW rats accepted WF skin grafts in 50% of grafted animals. Analysis of this phenomenon revealed that the adoptive transfer procedure itself was important in achieving skin allograft acceptance by these animals. In general, it seems that the lack of ability of irradiated and reconstituted animals to accept fully MHC disparate skin grafts results from the inability of these animals to suppress lymph node effector cells against I region antigen seen on highly immunogenic allogeneic Langerhans cells in the skin.
- Research Organization:
- Boston Univ., MA (USA). Graduate School
- OSTI ID:
- 5617596
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ANTIMETABOLITES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
BONE MARROW
CARDIOVASCULAR SYSTEM
DRUGS
EXTERNAL IRRADIATION
GRAFTS
HEART
HEMATOPOIETIC SYSTEM
IMMUNOLOGY
IMMUNOSUPPRESSION
IRRADIATION
KIDNEYS
LETHAL IRRADIATION
MAMMALS
METHOTREXATE
MICE
ORGANS
RADIATION EFFECTS
RADIOINDUCTION
RATS
RODENTS
SKIN
SOMATIC CELLS
SPLEEN CELLS
TISSUES
TRANSPLANTS
VERTEBRATES
WHOLE-BODY IRRADIATION
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ANTIMETABOLITES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
BONE MARROW
CARDIOVASCULAR SYSTEM
DRUGS
EXTERNAL IRRADIATION
GRAFTS
HEART
HEMATOPOIETIC SYSTEM
IMMUNOLOGY
IMMUNOSUPPRESSION
IRRADIATION
KIDNEYS
LETHAL IRRADIATION
MAMMALS
METHOTREXATE
MICE
ORGANS
RADIATION EFFECTS
RADIOINDUCTION
RATS
RODENTS
SKIN
SOMATIC CELLS
SPLEEN CELLS
TISSUES
TRANSPLANTS
VERTEBRATES
WHOLE-BODY IRRADIATION