Skip to main content
U.S. Department of Energy
Office of Scientific and Technical Information

Lead determination in blood by atomic absorption spectroscopy

Journal Article · · Am. Ind. Hyg. Assoc. J.; (United States)
A one-tube sample preparation method for blood lead determination which is relatively simple to perform and which does not require background correction for matrix effects is described. The red cells are lysed with a dilute potassium cyanide solution and heat. A citrate buffer is added, and the solution is then extracted with ammonium pyrrolidine dithiocarbamate and methyl isobutyl ketone. An aiquot of the MIBK layer is applied to a carbon rod atomizer and the peak at 217.0 nm is measured. The method demonstrates an overal in-run reproducibility of 1.39 ..mu..g/100 mL blood standard deviation, a between-run standard deviation of 2.27 ..mu..g/100 mL blood and a sensitivity of 0.012 ..mu..g/mL/1% nm.
Research Organization:
Gates Rubber Co., Denver, CO
OSTI ID:
5617210
Journal Information:
Am. Ind. Hyg. Assoc. J.; (United States), Journal Name: Am. Ind. Hyg. Assoc. J.; (United States) Vol. 40:12; ISSN AIHAA
Country of Publication:
United States
Language:
English