Polymer immobilized enzyme optrodes for the detection of penicillin
The preparation and performance of two enzyme-based fiber-optic sensors (optrodes) capable of detecting penicillin are described. Each sensor consists of a polymer membrane that is covalently attached to the tip of a glass optical fiber. The membrane contains the enzyme penicillinase and a pH-sensitive fluorescent dye. A signal is produced when the enzyme catalyzes the cleavage of the ..beta..-lactam ring of penicillin to produce penicilloic acid and, consequently, a pH change in the microenvironment of the membrane. The sensors differ in the way the polymer membrane is constructed and in the type of pH indicator dye used. Both optrodes exhibit response times (40-60 s) significantly lower than those of the corresponding enzyme electrodes (2 min). Each gives a linear response over the concentration range of 0.00025 to 0.01 M penicillin G, when measured in a 0.005 M phosphate buffer. The data indicate that these immobilization strategies produce similar results and may be considered complementary alternatives in future enzyme optrode applications.
- Research Organization:
- Lawrence Livermore National Lab., CA
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 5616498
- Journal Information:
- Anal. Chem.; (United States), Vol. 59:24
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
PENICILLIN
ABSORPTION SPECTROSCOPY
ENZYMES
EXPERIMENTAL DATA
FIBER OPTICS
MEMBRANES
PH VALUE
POLYMERS
QUANTITATIVE CHEMICAL ANALYSIS
TRACE AMOUNTS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
CHEMICAL ANALYSIS
DATA
DRUGS
INFORMATION
NUMERICAL DATA
SPECTROSCOPY
400102* - Chemical & Spectral Procedures