Proton translocation by cytochrome oxidase vesicles catalyzing the peroxidatic oxidation of ferrocytochrome c

Journal Article · Tue Mar 25 04:00:00 EST 1986 · J. Biol. Chem.; (United States)

OSTI ID:5615344

Miki, T; Orii, Y

Coupled with the peroxidatic oxidation of ferrocytochrome c under anaerobic conditions, proteoliposomes reconstituted with a purified preparation of bovine heart cytochrome oxidase ejected protons into the external medium with an apparent H+/e- ratio of 0.9. At the same time, protons in the intravesicular space were consumed. Dicyclohexylcarbodiimide significantly inhibited the proton translocation. Cyanide (0.14 mM) completely inhibited both the peroxidase and proton translocating activities. On the contrary, in the presence of 1 mM CO the proton ejection was abolished almost completely, but 50% of the peroxidase activity persisted. This result suggests the operation of multiple mechanisms in the peroxidase reaction and that the CO-sensitive one is coupled to the proton translocation.

• Research Organization: Kyoto Univ., Japan
• OSTI ID: 5615344
• Journal Information: J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 9; ISSN JBCHA
• Country of Publication: United States
• Language: English

Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BARYONS
BIOLOGICAL EFFECTS
BODY
CARBON COMPOUNDS
CARBON MONOXIDE
CARBON OXIDES
CARDIOVASCULAR SYSTEM
CATTLE
CELL CULTURES
CHALCOGENIDES
CHEMICAL REACTIONS
CYANIDES
CYTOCHROME OXIDASE
CYTOCHROMES
DOMESTIC ANIMALS
ELEMENTARY PARTICLES
ENZYMES
FERMIONS
HADRONS
HAEM DEHYDROGENASES
HEART
MAMMALS
MUSCLES
MYOCARDIUM
NUCLEONS
ORGANIC COMPOUNDS
ORGANS
OXIDATION
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PEROXIDES
PIGMENTS
PROTEINS
PROTONS
RUMINANTS
TRANSLOCATION
VERTEBRATES