Four-dimensional sup 13 C/ sup 13 C-edited nuclear Overhauser Enhancement Spectroscopy of a protein in solution: Application to interleukin 1. beta

Journal Article · Tue Jan 01 00:00:00 EST 1991 · Biochemistry; (USA)

DOI:https://doi.org/10.1021/bi00215a002· OSTI ID:5613529

Clore, G M; Kay, L E; Bax, A; Gronenborn, A M ^[1]

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1. National Institutes of Health, Bethesda, MD (USA)

A four-dimensional {sup 13}C/{sup 13}C-edited NOESY experiment is described which dramatically improves the resolution of protein NMR spectra and enables the straightforward assignment of nuclear Overhauser effects involving aliphatic and/or aromatic protons in larger proteins. The experiment is demonstrated for uniformly (>95{percent}) {sup 13}C-labeled interleukin 1{beta}, a protein of 153 residues and 17.4 kDa, which plays a key role in the immune response. NOEs between aliphatic and/or aromatic protons are first spread out into a third dimension by the {sup 13}C chemical shift of the carbon atom attached to the originating proton and subsequently into a fourth dimension by the {sup 13}C chemical shift of the carbon atom attached to the destination proton. Thus, each NOE cross peak is labeled by four chemical shifts. By this means, ambiguities in the assignment of NOEs that arise from chemical shift overlap and degeneracy are completely removed. Further, NOEs between protons with the same chemical shifts can readily be detected providing their attached carbon atoms have different {sup 13}C chemical shifts. The design of the pulse sequence requires special care to minimize the level of artifacts arising from undesired coherence transfer pathways, and in particular those associated with diagonal peaks which correspond to magnetization that has not been transferred from one proton to another. The 4D {sup 13}C/{sup 13}C-edited NOESY experiment is characterized by high sensitivity as the through-bond transfer steps involve the large {sup 1}J{sub CH} (130 Hz) couplings, and it is possible to obtain high-quality spectra on 1-2 mM samples of {sup 13}C-labeled protein in as little as 3 days. This experiment should open up the application of protein structure determination by NMR to a large number of medium-sized proteins (150-300 residues) of biological interest.

• OSTI ID: 5613529
• Journal Information: Biochemistry; (USA), Vol. 30:1; ISSN 0006-2960
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
LYMPHOKINES
NUCLEAR MAGNETIC RESONANCE
PROTEIN STRUCTURE
GEOMETRY
AQUEOUS SOLUTIONS
CARBON 13
CHEMICAL SHIFT
ESCHERICHIA COLI
IMMUNE REACTIONS
OVERHAUSER EFFECT
BACTERIA
CARBON ISOTOPES
DISPERSIONS
EVEN-ODD NUCLEI
GROWTH FACTORS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MATHEMATICS
MICROORGANISMS
MITOGENS
MIXTURES
NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RESONANCE
SOLUTIONS
STABLE ISOTOPES
550601* - Medicine- Unsealed Radionuclides in Diagnostics