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Importance of phytoalexin tolerance and detoxification for pathogenicity. Progress report, June 1983-June 1985

Technical Report ·
OSTI ID:5613237
;

This study focuses on the biochemistry of phytoalexin detoxifying enzymes. Progress is reported on purification, substrate specificity, and mechanism of several enzymes involved. Some aspects of the regulation of pisatin demethylase were studied since parallel genetic studies suggest its regulation is an important factor in the pathogenicity of Nectria haematococca. We have established that the detoxification of pisatin requires two components: NADPH-cytochrome c reductase and cytochrome P-450. The ability to separate and reconstitute these two components has allowed us to demonstrate that the reductase from a non-demethylating isolate of N. haematococca will support demethylating activity when combined with the cytochrome P-450 from a demethylating isolate. The finding that the critical genetic factor here is the cytochrome P-450 fraction reinforces the hypothesis that the multiple pda genes of this fungus encode different cytochrome P-450 isozymes. The evidence for monooxygenase catalyzed metabolism of maackiain suggests the existence of additional members of a phytoalexin detoxifying cytochrome P-450 family. 4 refs., 1 fig., 1 tab.

Research Organization:
Cornell Univ., Ithaca, NY (USA). Dept. of Plant Pathology
DOE Contract Number:
AC02-83ER13073
OSTI ID:
5613237
Report Number(s):
DOE/ER/13073-T2; ON: DE85014759
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
553001 -- Agriculture & Food Technology-- Tracer Techniques-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
ALKALOIDS
ANTIGENS
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
CARBON 14 COMPOUNDS
CHEMISTRY
CYTOCHROMES
DISEASES
ENZYME ACTIVITY
ENZYMES
FRACTIONATION
FUNGAL DISEASES
GENES
INFECTIOUS DISEASES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LEGUMINOSAE
MATERIALS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PIGMENTS
PISUM
PLANTS
REACTION KINETICS
SEPARATION PROCESSES
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES