Molecular analysis of chromosomal rearrangements using pulsed field gel electrophoresis and somatic cell hybrids
Journal Article
·
· Environmental and Molecular Mutagenesis; (United States)
- Florida Inst. of Tech., Melbourne (United States)
Many human genetic diseases, including some cancers, are characterized by consistent chromosome abnormalities, such as deletions and translocations. Analyses of these mutations often prove crucial to the eventual cloning and characterization of the gene(s) responsible for the disease. Two methods for analyzing these chromosome abnormalities have been developed in recent years: somatic cell hybridization and pulsed field gel electrophoresis (PFGE). Somatic cell hybridization is a technique for segregating an aberrant chromosome from its normal homologue in a cell derived from an unrelated species, which is usually a rodent. Demonstrations of these analytic techniques are presented, using as an example chromosomal abnormalities involving human chromosome band 11p13, the locus for the Wilms' tumor, aniridia, genitourinary abnormality, and mental retardation (WAGR) syndrome.
- OSTI ID:
- 5610651
- Journal Information:
- Environmental and Molecular Mutagenesis; (United States), Journal Name: Environmental and Molecular Mutagenesis; (United States) Vol. 18:4; ISSN EMMUE; ISSN 0893-6692
- Country of Publication:
- United States
- Language:
- English
Similar Records
Submicroscopic deletions at the WAGR locus, revealed by nonradioactive in situ hybridization
A 1.7-Mb YAC contig around the human BDNF gene (11p13): integration of the physical, genetic, and cytogenetic maps in relation to WAGR syndrome
Tumor-specific loss of 11p15. 5 alleles in del11p13 Wilms tumor and in familial adrenocortical carcinoma
Journal Article
·
Mon Nov 30 23:00:00 EST 1992
· American Journal of Human Genetics; (United States)
·
OSTI ID:6707564
A 1.7-Mb YAC contig around the human BDNF gene (11p13): integration of the physical, genetic, and cytogenetic maps in relation to WAGR syndrome
Journal Article
·
Mon Oct 31 23:00:00 EST 1994
· Genomics
·
OSTI ID:255219
Tumor-specific loss of 11p15. 5 alleles in del11p13 Wilms tumor and in familial adrenocortical carcinoma
Journal Article
·
Mon May 01 00:00:00 EDT 1989
· Proceedings of the National Academy of Sciences of the United States of America; (USA)
·
OSTI ID:5160066
Related Subjects
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL MARKERS
BODY
CHROMOSOMAL ABERRATIONS
CHROMOSOMES
CLONING
DISEASES
DNA HYBRIDIZATION
DNA-CLONING
ELECTROPHORESIS
GENETIC MAPPING
HEREDITARY DISEASES
HETEROCHROMOSOMES
HYBRIDIZATION
KIDNEYS
MAPPING
MOLECULAR BIOLOGY
MUTATIONS
ORGANS
UROGENITAL SYSTEM DISEASES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL MARKERS
BODY
CHROMOSOMAL ABERRATIONS
CHROMOSOMES
CLONING
DISEASES
DNA HYBRIDIZATION
DNA-CLONING
ELECTROPHORESIS
GENETIC MAPPING
HEREDITARY DISEASES
HETEROCHROMOSOMES
HYBRIDIZATION
KIDNEYS
MAPPING
MOLECULAR BIOLOGY
MUTATIONS
ORGANS
UROGENITAL SYSTEM DISEASES