Androstenedione and testosterone biosynthesis by the adrenal cortex of the horse

Journal Article · Wed Feb 01 00:00:00 EST 1984 · Steroids; (United States)

DOI:https://doi.org/10.1016/0039-128X(84)90033-3· OSTI ID:5609654

Silberzahn, P; Rashed, F; Zwain, I; Leymarie, P

An homogenate from cortical tissue of mare adrenals was incubated in the presence of tritiated pregnenolone. The (/sup 3/H) androstenedione and the (/sup 3/H) testosterone synthesized during the incubation were extracted, purified, and co-crystallized to constant specific activity in the presence of unlabeled carriers. The rate of conversion of pregnenolone to androstenedione and testosterone was of the order of 5 and 0.15 per cent respectively. The high ratio of (/sup 3/H) androstenedione to (/sup 3/H) testosterone observed in this study suggests that androstenedione is the main androgen produced by mare adrenals. It is concluded that adrenals could contribute to the production of blood androgens in normal as well as hyperandrogenic mares.

• Research Organization: U.E.R. des Sciences Univ., Caen, France
• OSTI ID: 5609654
• Journal Information: Steroids; (United States), Vol. 43:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANDROSTENEDIONE
BIOSYNTHESIS
TESTOSTERONE
ADRENAL GLANDS
BLOOD
HORSES
ISOTOPE DILUTION
PURIFICATION
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANDROGENS
ANDROSTANES
ANIMALS
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
ENDOCRINE GLANDS
GLANDS
HORMONES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KETONES
LABELLED COMPOUNDS
MAMMALS
MATERIALS
ORGANIC COMPOUNDS
ORGANS
STEROID HORMONES
STEROIDS
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques