Depression of biliary glutathione excretion by chronic ethanol feeding in the rat

Journal Article · Mon Mar 12 04:00:00 EST 1984 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(84)90020-1· OSTI ID:5606650

Vendemiale, G; Jayatilleke, E; Shaw, S; Lieber, C S

The effects of chronic alcohol feeding on biliary glutathione excretion were studied in rats pair fed diets containing either ethanol (36% of total energy) or isocaloric carbohydrate for 4-6 weeks. An exteriorized biliary-duodenal fistula was established and total glutathione (GSH) and oxidized glutathione (GSSG) were measured. A significant decrease was observed in rats fed alcohol chronically compared to their pair fed controls in the biliary excretion of GSH (55.7 +/- 37.0 vs 243.1 +/- 29.0 ..mu..g/ml bile, p < 0.025) as well as biliary GSSG (12.5 +/- 5.0 vs 49.9 +/- 8.0 ..mu..g/ml bile, p < 0.05) and in bile flow (23.1 +/- 1.6 vs 29.2 +/- 1.3 ..mu..g/min, p < 0.05). An acute dose of ethanol tended to exaggerate the decrease on biliary GSH and GSSG in the two groups of animals. The depression in biliary GSH could not be attributed to decreased GSH synthesis since S/sup 35/-L-methionine incorporation into hepatic and biliary GSH was unchanged or even increased after chronic ethanol feeding. 22 references, 4 figures.

• Research Organization: Bronx VA Medical Center, New York, NY
• OSTI ID: 5606650
• Journal Information: Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 34:11; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
560305* -- Chemicals Metabolism &amp; Toxicology-- Vertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BILIARY TRACT
BIOCHEMICAL REACTION KINETICS
BODY
CARBOXYLIC ACIDS
CHRONIC INTAKE
CLEARANCE
DAYS LIVING RADIOISOTOPES
DIET
DIGESTIVE SYSTEM
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ETHANOL
EVEN-ODD NUCLEI
EXCRETION
GLANDS
GLUTATHIONE
HYDROXY COMPOUNDS
INHIBITION
INTAKE
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
LIPOTROPIC FACTORS
LIVER
MAMMALS
METHIONINE
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOISOTOPES
RADIOPROTECTIVE SUBSTANCES
RATS
REACTION KINETICS
RODENTS
SULFUR 35
SULFUR ISOTOPES
TOXICITY
TRACER TECHNIQUES
VERTEBRATES