Chromosomal localization of genes encoding guanine nucleotide-binding protein subunits in mouse and human

Journal Article · Sat Oct 01 04:00:00 EDT 1988 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.85.20.7642· OSTI ID:5606502

Blatt, C; Eversole-Cire, P; Cohn, V H; Zollman, S; Fournier, R E.K.; Mohandas, L T; Nesbitt, M; Lugo, T; Jones, D T; Reed, R R; Weiner, L P; Sparkes, R S; Simon, M I ^[1]

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1. Weizmann Institute, Rehovoth (Israel)

A variety of genes have been identified that specify the synthesis of the components of guanine nucleotide-binding proteins (G proteins). Eight different guanine nucleotide-binding {alpha}-subunit proteins, two different {beta} subunits, and one {gamma} subunit have been described. Hybridization of cDNA clones with DNA from human-mouse somatic cell hybrids was used to assign many of these genes to human chromosomes. The retinal-specific transducin subunit genes GNAT1 and GNAT2 were on chromosomes 3 and 1; GNAI1, GNAI2, and GNAI3 were assigned to chromosomes 7, 3, and 1, respectively; GNAZ and GNAS were found on chromosomes 22 and 20. The {beta} subunits were also assigned-GNB1 to chromosome 1 and GNB2 to chromosome 7. Restriction fragment length polymorphisms were used to map the homologues of some of these genes in the mouse. GNAT1 and GNAI2 were found to map adjacent to each other on mouse chromosome 9 and GNAT2 was mapped on chromosome 17. The mouse GNB1 gene was assigned to chromosome 19. These mapping assignments will be useful in defining the extend of the G{alpha} gene family and may help in attempts to correlate specific genetic diseases and with genes corresponding to G proteins.

• OSTI ID: 5606502
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 85:20; ISSN PNASA; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMALS
AROMATICS
AZAARENES
BIOSYNTHESIS
CHROMOSOMES
DNA
GENES
GENETIC MAPPING
GUANINE
HETEROCHROMOSOMES
HETEROCYCLIC COMPOUNDS
HUMAN CHROMOSOME 19
HYBRIDIZATION
HYDROXY COMPOUNDS
MAMMALS
MAN
MAPPING
MICE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PRIMATES
PROTEINS
PURINES
RECOMBINANT DNA
RFLPS
RODENTS
SYNTHESIS
VERTEBRATES