The biotransformation in vitro of cysteinyl leukotrienes in blood of normal and asthmatic subjects

Journal Article · Fri Mar 31 23:00:00 EST 1989 · Prostaglandins; (USA)

DOI:https://doi.org/10.1016/0090-6980(89)90093-2· OSTI ID:5606059

Zakrzewski, J T; Sampson, A P; Evans, J M; Barnes, N C; Piper, P J; Costello, J F ^[1]

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1. King's College School of Medicine and Dentistry, Denmark Hill, London (England)

The metabolism of exogenous leukotriene C4 (LTC4), LTD4 and LTE4 (10(-8) M) was studied in vitro in blood of normal and asthmatic subjects for up to 2 hr by reverse-phase high performance liquid chromatography. In whole blood, incubation of LTC4 (T1/2 = 11.5 min) resulted in the formation of LTD4 and LTE4 whose biosynthesis was inhibited by serine borate (30 mM). Similar experiments performed with LTD4 (T1/2 = 5 min) produced a single metabolite (LTE4) which was inhibited by L-cysteine (10 mM). On the other hand, LTE4 represented a highly stable product in our in vitro system. The bioconversion of LTC4 or LTD4 was slower in plasma but this effect appeared more pronounced for the cysteinylglycinyl derivative. The bioconversion of LTD4 in whole blood or plasma was almost twice as rapid as LTC4. Experiments performed with asthmatic blood showed no significant difference in the survival of LTC4. These results suggest that blood may play a role in regulating the bioavailability of cysteinyl-containing LTs which could be of relevance to their excretion in man.

• OSTI ID: 5606059
• Journal Information: Prostaglandins; (USA), Journal Name: Prostaglandins; (USA) Vol. 37:4; ISSN 0090-6980; ISSN PRGLB
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
550901 -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ASTHMA
BIOLOGICAL MATERIALS
BLOOD
BODY FLUIDS
CHROMATOGRAPHY
DISEASES
HYDROGEN COMPOUNDS
IN VITRO
ISOTOPE APPLICATIONS
ISOTOPE DILUTION
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MAN
MATERIALS
METABOLISM
METABOLITES
PATHOGENESIS
PRIMATES
PROSTAGLANDINS
RESPIRATORY SYSTEM DISEASES
SEPARATION PROCESSES
SURVIVAL TIME
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES