Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Drastically increased expression of MYC and FOS protooncogenes during in vitro differentiation of chronic lymphocytic leukemia cells

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
; ; ; ;
Chronic lymphocytic leukemia cells, representing a clonal population of resting B lymphocytes, were induced to differentiate into immunoglobulin-secreting lymphoblasts and plasmablasts by phorbol 12-myristate 13-acetate. The induction resulted in a rapid increase in the molar ratio of secreted/membrane-bound ..mu..-chain mRNA. Immunoglobulin secretion was preceded by a transition of the cells from the G/sub 0/ to G/sub 1/ phase of the cell cycle, as indicated by an increase in RNA and protein synthesis, and an overall increase in cellular RNA. The cells, however, became blocked in G/sub 1/ and did not enter S phase. The expression of MYC and FOS was rapidly induced by the phorbol 12-myristate 13-acetate treatment. MYC expression remained at a relatively high level during the whole differentiation process. It is thus concluded that a decline of MYC expression is not a prerequisite for differentiation of the chronic lymphocytic leukemia cells. This suggests that MYC expression may play a different role during differentiation of nonproliferating B cells than in the myelomonocytic cell lines HL-60 and U-937, where MYC expression has been reported to decrease during induced differentiation. The results also show that the expression of the MYC and FOS genes does not result in the transition of these cells into the S phase of the cell cycle.
Research Organization:
University Hospital, Uppsala, Sweden
OSTI ID:
5605782
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:1; ISSN PNASA
Country of Publication:
United States
Language:
English

Similar Records

Phorbol ester-induced growth arrest of murine myelomonocytic leukemic cells with virus-disrupted myb locus is not accompanied by decreased myc and myb expression
Journal Article · Wed Dec 31 23:00:00 EST 1986 · Proc. Natl. Acad. Sci. U.S.A.; (United States) · OSTI ID:5649257
Phorbol ester-induced terminal differentiation is inhibited in human U-937 monoblastic cells expressing a v-myc oncogene
Journal Article · Thu Mar 31 23:00:00 EST 1988 · Proc. Natl. Acad. Sci. U.S.A.; (United States) · OSTI ID:5982573
Decline in c-myc mRNA expression but not the induction of c-fos mRNA expression is associated with differentiation of SH-SY5Y human neuroblastoma cells
Journal Article · Mon Oct 31 23:00:00 EST 1988 · Experimental Cell Research; (United States) · OSTI ID:5322085

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
560300 -- Chemicals Metabolism & Toxicology
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
AZINES
BIOLOGICAL EFFECTS
CARCINOGENS
CELL DIFFERENTIATION
DISEASES
ESTERS
GENE REGULATION
GENES
GLOBULINS
HEMIC DISEASES
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IMMUNOASSAY
IMMUNOGLOBULINS
IMMUNOLOGY
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUKEMIA
NEOPLASMS
NUCLEOSIDES
NUCLEOTIDES
ONCOGENES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHORBOL ESTERS
PROTEINS
PYRIMIDINES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RIBOSIDES
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TUMOR CELLS
URACILS
URIDINE