Proton NMR study of a complex between the lac repressor headpiece and a 22 base pair symmetric lac operator

Journal Article · Tue Apr 04 00:00:00 EDT 1989 · Biochemistry; (USA)

DOI:https://doi.org/10.1021/bi00433a037· OSTI ID:5605599

Lamerichs, R M.J.N.; Boelens, R; van der Marel, G A; van Boom, J H; Kaptein, R; Buck, F; Fera, B; Rueterjans, H ^[1]

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1. Univ. of Utrecht (Netherlands)

A complex between the lac repressor headpiece and a fully symmetric tight-binding 22 bp lac operator was studied by 2D NMR. Several 2D NOE spectra were recorded for the complex in both H{sub 2}O and {sup 2}H{sub 2}O. Many NOE cross-peaks between the headpiece and DNA could be identified, and changes in the chemical shift of the DNA protons upon complex formation were analyzed. Comparison of these data with those obtained for a complex between the headpiece and a 14 bp half-operator, studied previously, shows that two headpieces form a specific complex with the 22 bp lac operator in which each headpiece binds in the same way as found for the 14 bp complex. The orientation of the recognition helix in the major groove of DNA in these complexes is opposite with respect to the dyad axis to that found for other repressors.

• OSTI ID: 5605599
• Journal Information: Biochemistry; (USA), Journal Name: Biochemistry; (USA) Vol. 28:7; ISSN 0006-2960; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
BACTERIA
BARYONS
CHEMICAL SHIFT
DNA
ELEMENTARY PARTICLES
ESCHERICHIA COLI
FERMIONS
GENE REGULATION
GENE REPRESSORS
HADRONS
HEAVY WATER
HYDROGEN COMPOUNDS
MAGNETIC RESONANCE
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEAR MAGNETIC RESONANCE
NUCLEIC ACIDS
NUCLEONS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
OVERHAUSER EFFECT
OXYGEN COMPOUNDS
PROTEINS
PROTONS
RESONANCE
WATER