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Modifiers of free radicals inhibit in vitro the oncogenic actions of x-rays, bleomycin, and the tumor promoter 12-O-tetradecanoylphorbol 13-acetate

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
;
Using short-term cultures of hamster embryo cells, we have examined the effects of the free-radical scavenger superoxide dismutase (superoxide:superoxide oxidoreductase, EC 1.15.1.1) and the enzyme catalase (hydrogen-peroxide:hydrogen-peroxide oxidoreductase, EC 1.11.1.6) on x-ray-and bleomycin-induced transformation and on the enhancement of radiogenic transformation by the tumor promoter 12-O-tetradecanoylphorbol 13-acetate (TPA). We find that superoxide dismutase inhibits (i) transformation induced by x-ray and bleomycin and (ii) promotional action of TPA in vitro. The results suggest that the oncogenic action of x-rays and bleomycin and the enhancement of oncogenic transformation by TPA are mediated in part by free radicals. The findings also suggest that superoxide dismutase can serve as an inhibitor of oncogenesis and that its actions, as seen in this in vitro system, are most predominantly on inhibiting late events in the progression of cellular transformation--those associated with promotion.
Research Organization:
Institute of Cancer Research, New York, NY
DOE Contract Number:
AC02-78EV04733
OSTI ID:
5605352
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 80:5; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
BIOLOGICAL EFFECTS
BLEOMYCIN
CELL CULTURES
CHEMICAL ACTIVATION
CHO CELLS
DRUGS
ELECTROMAGNETIC RADIATION
ENZYMES
IN VITRO
INHIBITION
IONIZING RADIATIONS
ONCOGENIC TRANSFORMATION
ONCOGENIC TRANSFORMATIONS
OXIDOREDUCTASES
PROMOTERS
RADIATIONS
RADICALS
RADIOINDUCTION
RESPONSE MODIFYING FACTORS
SUPEROXIDE RADICALS
TUMOR PROMOTERS
X RADIATION