Molecular cloning of a putative receptor protein kinase gene encoded at the self-incompatibility locus of Brassica oleracea
- Cornell Univ., Ithaca, NY (United States)
Self-recognition between pollen and stigma during pollination in Brassica oleracea is genetically controlled by the multiallelic self-incompatibility locus (S). The authors describe the S receptor kinase (SRK) gene, a previously uncharacterized gene that residues at the S locus. The nucleotide sequences of genomic DNA and of cDNAs corresponding to SRK predict a putative transmembrane receptor having serine/threonine-specific protein kinase activity. Its extracellular domain exhibits striking homology to the secreted product of the S-locus genotypes are highly polymorphic and have apparently evolved in unison with genetically linked alleles of SLG. SRK directs the synthesis of several alternative transcripts, which potentially encode different protein products, and these transcripts were detected exclusively in reproductive organs. The identification of SRK may provide new perspectives into the signal transduction mechanism underlying pollen recognition.
- DOE Contract Number:
- FG02-88ER13909
- OSTI ID:
- 5604490
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 88:19; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
GENES
DNA-CLONING
PHOSPHOTRANSFERASES
GENE REGULATION
BRASSICA
DNA HYBRIDIZATION
DNA SEQUENCING
OLIGONUCLEOTIDES
PHOSPHORUS 32
POLLEN
RECEPTORS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CLONING
DAYS LIVING RADIOISOTOPES
ENZYMES
FOOD
GAMETES
GERM CELLS
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MEMBRANE PROTEINS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PLANTS
PROTEINS
RADIOISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
VEGETABLES
550200* - Biochemistry