Autoradiographic quantification of vasoactive intestinal peptide binding sites in sections from human blood mononuclear cell pellets

Journal Article · Thu Sep 01 00:00:00 EDT 1988 · Neuropsychopharmacology; (United States)

DOI:https://doi.org/10.1016/0893-133X(88)90025-5· OSTI ID:5604268

Gutkind, J S; Kurihara, M; Castren, E; Saavedra, J M

Quantitative autoradiographic methods were utilized to characterize specific, high-affinity vasoactive intestinal peptide binding sites (Kd = 310 +/- 60 pmol/L; Bmax = 93 +/- 11 fmol/mg protein) in frozen sections obtained from a mononuclear cell pellet derived from 20 ml of human blood. The method is at least one order of magnitude more sensitive than conventional membrane binding techniques, and it has the potential for wide applications in studies of neuropeptide, biogenic amine, and drug binding in clinical samples.

• Research Organization: National Institute of Mental Health, Bethesda, MD (USA)
• OSTI ID: 5604268
• Journal Information: Neuropsychopharmacology; (United States), Vol. 1:3
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PEPTIDES
RECEPTORS
AUTORADIOGRAPHY
BIOCHEMICAL REACTION KINETICS
CHEMICAL COMPOSITION
IN VITRO
IODINE ISOTOPES
MAN
MONOCYTES
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
ISOTOPES
KINETICS
LEUKOCYTES
MAMMALS
MATERIALS
MEMBRANE PROTEINS
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
REACTION KINETICS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques