Distribution of vasoactive intestinal polypeptide and substance P receptors in human colon and small intestine

Journal Article · Sat Jul 01 00:00:00 EDT 1989 · Dig. Dis. Sci.; (United States)

DOI:https://doi.org/10.1007/BF01536382· OSTI ID:5603541

Korman, L Y; Sayadi, H; Bass, B; Moody, T W; Harmon, J W

Vasoactive intestinal polypeptide (VIP) and substance P are found in neurons in the lamina propria and submucosa and muscularis propria of human small intestine and colon. VIP receptors coupled to adenylate cyclase are present on epithelial, smooth muscle, and mononuclear cells. This study analyzes the distribution of (/sup 125/I)VIP binding and (/sup 125/I)substance P in human colon and small intestine using autoradiographic techniques. (/sup 125/I)VIP binding was present in high density in the mucosal layer of colon and small intestine. (/sup 125/I)VIP binding was not significantly greater than nonspecific binding in smooth muscle layers or the lymphoid follicles. In contrast, (/sup 125/I)substance P binding was present in high density over the colonic muscle but was not present over the mucosal layer. In human colon cancer, (/sup 125/I)VIP grain density over the malignant tissue was only slightly higher than background. These autoradiographic studies of (/sup 125/I)VIP binding indicate that the highest density of VIP receptors was found in the small intestine and superficial colonic mucosa, whereas the density of substance P receptors was highest over the smooth muscle layers. These findings suggest a mismatch between immunochemical content of the peptide and autoradiographic density of the receptor.

• Research Organization: Veterans Administration Medical Center, Washington, DC (USA)
• OSTI ID: 5603541
• Journal Information: Dig. Dis. Sci.; (United States), Journal Name: Dig. Dis. Sci.; (United States) Vol. 34:7; ISSN DDSCD
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
CYCLASES
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISEASES
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
GASTROINTESTINAL TRACT
INTERMEDIATE MASS NUCLEI
INTESTINES
IODINE 125
IODINE ISOTOPES
ISOTOPES
KINETICS
LARGE INTESTINE
LYASES
MAMMALS
MAN
MEMBRANE PROTEINS
MEMBRANES
MUCOUS MEMBRANES
MUSCLES
NEOPLASMS
NERVE CELLS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
POLYPEPTIDES
PRIMATES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
SOMATIC CELLS
TISSUE DISTRIBUTION
VERTEBRATES