The phenotype of Arabidopsis thaliana det1 mutants suggest a role for cytokinins in greening
When grown in the absence of light, the det1 mutants of Arabidopsis thaliana develop characteristics of light-grown plants by morphological, cellular, and molecular criteria. Further, in light-grown plants, mutations in the DET1 gene affect cell-type-specific expression of light-regulated genes and the chloroplast developmental program. Here we show that the addition of exogenously added cytokinins (either 2-isopentenyl adenine, kinetin, or benzyladenine) to the growth medium of dark-germinated wild-type seedlings results in seedlings that resemble det1 mutants, instead of having the normal etiolated morphology. Like det1 mutants, these dark-grown seedlings now contain chloroplasts and have high levels of expression of genes that are normally light''-regulated. These results suggest an important role for cytokinins during greening of Arabidopsis, and may implicate cytokinin levels or an increased sensitivity to cytokinins as explanations for some of the observed phenotypes of det1 mutants.
- Research Organization:
- Salk Inst. for Biological Studies, San Diego, CA (United States)
- Sponsoring Organization:
- DOE; NSF; SRNFOUN; ELEINST; USDOE, Washington, DC (United States); National Science Foundation, Washington, DC (United States); Samuel Roberts Noble Foundation, Ardmore, OK (United States); Elementary Inst. of Science, San Diego, CA (United States)
- DOE Contract Number:
- FG03-89ER13993
- OSTI ID:
- 5603534
- Report Number(s):
- DOE/ER/13993-T1; ON: DE92007058
- Country of Publication:
- United States
- Language:
- English
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