Room temperature single molecule microscopes
Conference
·
OSTI ID:560335
- Los Alamos National Lab., NM (United States)
We have developed three capabilities to image the locations of and interrogate immobilized single fluorescent molecules: near-field scanning optical, confocal scanning optical, and wide-field epi-fluorescence microscopy. Each microscopy has its own advantages. Near-field illumination can beat the diffraction limit. Confocal microscopy has high brightness and temporal resolution. Wide-field has the quickest (parallel) imaging capability. With confocal microscopy, we have verified that single fluorescent spots in our images are due to single molecules by observing photon antibunching. Using all three microscopies, we have observed that xanthene molecules dispersed on dry silica curiously exhibit intensity fluctuations on millisecond to minute time scales. We are exploring the connection between the intensity fluctuations and fluctuations in individual photophysical parameters. The fluorescence lifetimes of Rhodamine 6G on silica fluctuate. The complex nature of the intensity and lifetime fluctuations is consistent with a mechanism that perturbs more than one photophysical parameter.
- OSTI ID:
- 560335
- Report Number(s):
- CONF-970443--
- Country of Publication:
- United States
- Language:
- English
Similar Records
Fluorescence detection of single molecules using pulsed near-field optical excitation and time correlated photon counting
Imaging biological molecules with single molecule sensitivity using near-field scanning optical microscopy
Alterations of single molecule fluorescence lifetimes in near-field optical microscopy
Conference
·
Mon Feb 28 23:00:00 EST 1994
·
OSTI ID:10129372
Imaging biological molecules with single molecule sensitivity using near-field scanning optical microscopy
Conference
·
Thu Nov 30 23:00:00 EST 1995
·
OSTI ID:151800
Alterations of single molecule fluorescence lifetimes in near-field optical microscopy
Journal Article
·
Fri Jul 15 00:00:00 EDT 1994
· Science (Washington, D.C.); (United States)
·
OSTI ID:6768949