sup 1 H and sup 15 N resonance assignments of oxidized flavodoxin from Anacystis nidulans with 3D NMR

Journal Article · Tue Aug 06 00:00:00 EDT 1991 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00245a008· OSTI ID:5602898

Clubb, R T ^[1]; Thanabal, V; Wagner, G ^[2]; Osborne, C ^[3]

---

1. Univ. of Michigan, Ann Arbor (United States) Harvard Medical School, Boston, MA (United States)
2. Univ. of Michigan, Ann Arbor (United States)
3. Harvard Medical School, Boston, MA (United States)

Proton and nitrogen-15 sequence-specific nuclear magnetic resonance assignments have been determined for recombinant oxidized flavodoxin from Anacystis nidulans. Assignments were obtained by using {sup 15}N-{sup 1}H heteronuclear three-dimensional (3D) NMR spectroscopy on a uniformly nitrogen-15 enriched sample of the protein, pH 6.6, at 30C. For 165 residues, the backbone and a large fraction of the side-chain proton resonances have been assigned. Medium- and long-range NOE's have been used to characterize the secondary structure. In solution, flavodoxin consists of a five-stranded parallel {beta} sheet involving residues 3-9, 31-37, 49-56, 81-89, 114-117, and 141-144. Medium-range NOE's indicate that presence of several helices. Several {sup 15}N and {sup 1}H resonances of the flavin mononucleotide (FMN) prosthetic group have been assigned. The FMN-binding site has been investigated by using polypeptide-FMN NOE's.

• OSTI ID: 5602898
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 30:31; ISSN 0006-2960; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACRIDINES
AMINES
AROMATICS
AZAARENES
AZINES
BARYONS
CHEMICAL REACTIONS
CHEMICAL SHIFT
ELEMENTARY PARTICLES
ENZYMES
FERMIONS
FLAVINES
HADRONS
HETEROCYCLIC COMPOUNDS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
NITROGEN 15
NITROGEN ISOTOPES
NUCLEAR MAGNETIC RESONANCE
NUCLEI
NUCLEONS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OVERHAUSER EFFECT
OXIDATION
PROTEINS
PROTONS
PYRIDINES
RESONANCE
STABLE ISOTOPES
TRANSFERASES