Use of 8-methoxypsoralen and long-wavelength ultraviolet radiation for decontamination of platelet concentrates

Journal Article · Sat Jul 01 04:00:00 EDT 1989 · Blood; (USA)

OSTI ID:5601855

Lin, L; Wiesehahn, G P; Morel, P A; Corash, L ^[1]

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1. Diamond Scientific Company, Des Moines, IA (USA)

Transmission of viral diseases through blood products remains an unsolved problem in transfusion medicine. We have developed a psoralen photochemical system for decontamination of platelet concentrates in which platelets are treated with long wavelength ultraviolet radiation (UVA, 320-400 nm) in the presence of 8-methoxypsoralen (8-MOP). Bacteria, RNA viruses, and DNA viruses ranging in genome size from 1.2 x 10(6) daltons, encompassing the size range of human pathogens, were inoculated into platelet concentrates and subjected to treatment. This system inactivated 25 to 30 logs/h of bacteria Escherichia coli or Staphylococcus aureus, 6 logs/h of bacteriophage fd, 0.9 log/h of bacteriophage R17 and 1.1 logs/h of feline leukemia virus (FeLV) in platelet concentrates maintained in standard storage bags. Platelet integrity and in vitro function before, immediately following photochemical treatment, and during prolonged storage after treatment, were evaluated by measuring: (1) extracellular pH; (2) platelet yields; (3) extracellular lactate dehydrogenase (LDH) levels; (4) platelet morphology; (5) platelet aggregation responsiveness; (6) thromboxane beta-2 (TXB-2) production; (7) dense body secretion; and (8) alpha granule secretion. These assays demonstrated that this photochemical inactivation system inactivated bacteria and viruses in platelet concentrates with minimal adverse effects on the in vitro function of platelets in comparison to untreated control concentrates maintained under current, standard blood bank conditions.

• OSTI ID: 5601855
• Journal Information: Blood; (USA), Journal Name: Blood; (USA) Vol. 74:1; ISSN BLOOA; ISSN 0006-4971
• Country of Publication: United States
• Language: English

Related Subjects

560130* -- Radiation Effects on Microorganisms
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ANTICOAGULANTS
BACTERIA
BACTERIOPHAGES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BLOOD COAGULATION
BLOOD PLATELETS
BODY FLUIDS
CHEMISTRY
CLEANING
COUMARINS
DECONTAMINATION
DISEASES
DRUGS
ELECTROMAGNETIC RADIATION
ENZYMES
ESCHERICHIA COLI
HEMATOLOGIC AGENTS
HEMIACETAL DEHYDROGENASES
HETEROCYCLIC COMPOUNDS
INFECTIOUS DISEASES
LACTATE DEHYDROGENASE
LEUKEMIA VIRUSES
MAMMALS
MAN
MATERIALS
MICROORGANISMS
MORPHOLOGY
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
OXIDOREDUCTASES
PARASITES
PHOTOCHEMISTRY
PRIMATES
PROSTAGLANDINS
PSORALEN
RADIATION EFFECTS
RADIATIONS
STAPHYLOCOCCUS
STORAGE
ULTRAVIOLET RADIATION
VERTEBRATES
VIRAL DISEASES
VIRUSES