Labeling of monoclonal antibodies with samarium-153 for combined radioimmunoscintigraphy and radioimmunotherapy

Journal Article · Mon May 01 04:00:00 EDT 1989 · J. Nucl. Med.; (United States)

OSTI ID:5600618

Boniface, G R; Izard, M E; Walker, K Z; McKay, D R; Sorby, P J; Turner, J H; Morris, J G

The labeling of a monoclonal antibody K-1-21 with /sup 153/Sm has been investigated using the bifunctional chelate cyclic diethylenetriaminepentaacetic acid (DTPA) anhydride. Labeling efficiencies greater than 60% were obtained using high specific activity (/sup 153/Sm)chloride and a cDTPAa:MAb conjugation ratio of 20:1. The resultant labeled antibody had a s.a. greater than 150 MBq.mg-1 and a % retained immunoreactivity greater than 90%. Imaging and biodistribution studies in a rat model demonstrated that specific uptake of /sup 153/Sm-K-1-21 into s.c. implants of the target antigen could be clearly detected in scintigrams at 6 days p.i. The specific uptake (1.90 +/- 0.45% ID/g, 19.95 +/- 2.20 Implant:Blood ratio) compared favorably to /sup 131/I- and /sup 111/In-labeled K-1-21 (2.52 +/- 0.20 and 3.33 +/- 0.20% ID/g, 7.69 +/- 0.45 and 10.10 +/- 0.60 I:B, respectively). Labeling of MAbs with /sup 153/Sm for combined scintigraphy/therapy is feasible at clinically appropriate specific activities using cDTPAa, with the resultant conjugates retaining immunoreactivity and in vivo antigen localization.

• Research Organization: Univ. of Sydney (Australian)
• OSTI ID: 5600618
• Journal Information: J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 30:5; ISSN JNMEA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
550604 -- Medicine-- Unsealed Radionuclides in Therapy-- (1980-)
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINO ACIDS
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MODELS
CARBOXYLIC ACIDS
CHELATING AGENTS
COMPARATIVE EVALUATIONS
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DISTRIBUTION
DRUGS
DTPA
EFFICIENCY
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
IMMUNOLOGY
IMMUNOTHERAPY
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE 131
IODINE ISOTOPES
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLING
MAMMALS
MEDICINE
MINUTES LIVING RADIOISOTOPES
MONOCLONAL ANTIBODIES
NUCLEAR MEDICINE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
RADIOIMMUNOLOGY
RADIOIMMUNOSCINTIGRAPHY
RADIOIMMUNOTHERAPY
RADIOISOTOPE SCANNING
RADIOISOTOPES
RADIOLOGY
RADIOPROTECTIVE SUBSTANCES
RADIOTHERAPY
RARE EARTH ISOTOPES
RARE EARTH NUCLEI
RATS
RODENTS
SAMARIUM 153
SAMARIUM ISOTOPES
SCINTISCANNING
THERAPY
TISSUE DISTRIBUTION
VERTEBRATES