TNT metabolites in animal tissues

Technical Report · Sat Jun 01 00:00:00 EDT 1991

OSTI ID:5600478

Shugart, L R; Griest, W H; Tan, E; Guzman, C; Caton, J E; Ho, C -H; Tomkins, B A

Analyses for TNT and nine potential metabolites (TNT-related compounds) were made in deer, rabbit, and quail tissues (muscle and liver) taken from the Alabama Army Ammunition Plant (AAAP), Childersburg, Alabama. The listed TNT-related compounds are 2,4,6- trinitrotoluene (parent compound); 2,4-diamino-6-nitrotoluene; 2,6-diamino-4-nitrotoluene; 2-amino-4,6-dinitrotoluene; 4-amino-2,6- dinitrotoluene; 2,4,6-trinitrobenzyl alcohol; 2,4,6-trinitrobenzoic acid; 1,3,5-trinitrobenzene; 4-hydroxylamino-2,6-dinitrotoluene; and 2,6,2',6'-tetranitro-4,4'-azoxytoluene. The procedure for extraction of these compounds from animal tissue required homogenization in acetonitrile, and subsequent partitioning into chloroform. Quantitative determination of extracted compounds was obtained by chromatographic separation on a mixed-mode HPLC column in which the phase bonded to the silica surface contained both a C18 (reversed-phase function) and a secondary amine (anion exchange function) incorporated into a single ligand. A ternary mobile phase gradient containing pH 5.1 phosphate buffer, methanol, and acetonitrile was used in separation. An experimental verification of the metabolism of TNT and the detection (or absence) of the selected metabolites was performed in mice subacutely dosed with 100 milligrams per kilogram of ({sup 14}C)-TNT. These studies show that the TNT-related compounds of concern do accumulate in muscle and liver tissue of the mouse under the experimental conditions imposed, but at concentrations below the 1.2 ppM level. However, products other than TNT and free metabolites may be accumulating since some ({sup 14}C) was found to be nonextractable. 13 refs., 5 figs., 6 tabs.

• Research Organization: Oak Ridge National Lab., TN (United States)
• Sponsoring Organization: USDOD; Department of Defense, Washington, DC (United States)
• DOE Contract Number: AC05-84OR21400
• OSTI ID: 5600478
• Report Number(s): ORNL/M-1336; ON: DE91015518
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
ANIMAL TISSUES
QUANTITATIVE CHEMICAL ANALYSIS
METABOLITES
TNT
BIOLOGICAL EFFECTS
CARBON 14 COMPOUNDS
DECISION TREE ANALYSIS
DEER
EXTRACTION
FOWL
LIQUID COLUMN CHROMATOGRAPHY
MICE
PROGRESS REPORT
RABBITS
ANIMALS
BIRDS
BODY
CARBON COMPOUNDS
CHEMICAL ANALYSIS
CHEMICAL EXPLOSIVES
CHROMATOGRAPHY
DOCUMENT TYPES
EXPLOSIVES
LABELLED COMPOUNDS
MAMMALS
NITRO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
RODENTS
RUMINANTS
SEPARATION PROCESSES
TISSUES
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550500 - Metabolism