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Technical Report · Sun Jan 01 04:00:00 EST 1989

DOI:https://doi.org/10.2172/5599513· OSTI ID:5599513

McCormick, J. J.

We have developed an infinite lifespan human fibroblast cell line after transfection of a v-{und myc} gene into human fibroblasts. The cells have normal morphology, have doubled more than 200 times since going through crisis, are not tumorigenic, and except for their infinite lifespan, are apparently normal. These cells can be converted into malignant cells by transfection and expression of a H-, N- or K-{und ras} oncogene. When {und finite} lifespan human fibroblasts are transfected with the same {und ras} gene constructions, the cells take on the same in vitro characteristics, but do not form tumors and they still retain the finite lifespan characteristic. Since the {und ras} and {und myc} oncogenes used to transfect the MSU-1.1 cells have normal proto-oncogene homologs in the human genome, it seems likely that it is these normal genes that become activated'' by carcinogen attack. We have undertaken the transfection studies to understand the role of various oncogenes in malignant transformation of human fibroblasts. With a knowledge of the phenotype caused by a specific oncogene, we can replace the transfection of a specific oncogene with carcinogen activation of the homologous gene. By proceeding in this way, we should be able to develop qualitative methods for the malignant transformation of human fibroblasts by carcinogens. 3 refs., 2 figs., 1 tab.

• Research Organization: Michigan State Univ., East Lansing, MI (USA)
• Sponsoring Organization: DOE/ER; DOHHS
• DOE Contract Number: FG02-87ER60524
• OSTI ID: 5599513
• Report Number(s): DOE/ER/60524-2; ON: DE90001524
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
CARCINOGENESIS
CELL PROLIFERATION
CELL TRANSFORMATIONS
CONNECTIVE TISSUE CELLS
DNA
DOCUMENT TYPES
FIBROBLASTS
GROWTH FACTORS
MAMMALS
MAN
MITOGENS
MOLECULAR BIOLOGY
MUTAGENESIS
NUCLEIC ACIDS
ONCOGENIC TRANSFORMATIONS
ORGANIC COMPOUNDS
PATHOGENESIS
PRIMATES
PROGRESS REPORT
PROTEINS
SOMATIC CELLS
VERTEBRATES