A fluorescence study of liposomes entrapped in sol-gel materials
- Sandia National Labs., Albuquerque, NM (United States)
Liposomes of phosphatidylcholine lipids were successfully entrapped in silicates using the sol-gel method with complete retention of the molecular aggregates over long periods in aqueous solution. Fluorescent studies of the small unilamellar vesicles of 5% pyrene labeled lipid PSIDA with DSPC remobilized in the gel found significant lipid reorganization upon aging in aqueous solutions. Monitoring of pyrene excimer (470 nm) to monomer (375 nm) ratios in the bilayer reveals that the silicate matrix tends to disperse PSIDA lipid aggregates from that observed in free solution. On an interesting note, the liposomes in the gel at pH 7.5. The PSIDA/DSPC liposomes, sensitive to heavy metal ions in free solution, maintain similar sensitivity in the gel yet the sensor material can not be recycled.
- Research Organization:
- Sandia National Laboratory
- DOE Contract Number:
- AC04-94AL85000
- OSTI ID:
- 559943
- Report Number(s):
- CONF-970443--
- Country of Publication:
- United States
- Language:
- English
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