Molecular cloning of a mouse DNA repair gene that complements the defect of group-A xeroderma pigmentosum

Journal Article · Sat Jul 01 00:00:00 EDT 1989 · Proc. Natl. Acad. Sci. USA; (United States)

DOI:https://doi.org/10.1073/pnas.86.14.5512· OSTI ID:5597192

Tanaka, K; Satokata, I; Ogita, Z; Uchida, T; Okada, Y

For isolation of the gene responsible for xeroderma pigmentosum (XP) complementation group A, plasmid pSV2gpt and genomic DNA from a mouse embryo were cotransfected into XP2OSSV cells, a group-A XP cell line. Two primary UV-resistant XP transfectants were isolated from about 1.6 X 10(5) pSV2gpt-transformed XP colonies. pSV2gpt and genomic DNA from the primary transfectants were again cotransfected into XP2OSSV cells and a secondary UV-resistant XP transfectant was obtained by screening about 4.8 X 10(5) pSV2gpt-transformed XP colonies. The secondary transfectant retained fewer mouse repetitive sequences. A mouse gene that complements the defect of XP2OSSV cells was cloned into an EMBL3 vector from the genome of a secondary transfectant. Transfections of the cloned DNA also conferred UV resistance on another group-A XP cell line but not on XP cell lines of group C, D, F, or G. Northern blot analysis of poly(A)+ RNA with a subfragment of cloned mouse DNA repair gene as the probe revealed that an approximately 1.0 kilobase mRNA was transcribed in the donor mouse embryo and secondary transfectant, and approximately 1.0- and approximately 1.3-kilobase mRNAs were transcribed in normal human cells, but none of these mRNAs was detected in three strains of group-A XP cells. These results suggest that the cloned DNA repair gene is specific for group-A XP and may be the mouse homologue of the group-A XP human gene.

• Research Organization: Osaka Univ. (Japan)
• OSTI ID: 5597192
• Journal Information: Proc. Natl. Acad. Sci. USA; (United States), Journal Name: Proc. Natl. Acad. Sci. USA; (United States) Vol. 86:14; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CONSTITUENTS
CLONING
DISEASES
DNA HYBRIDIZATION
DNA REPAIR
DNA REPLICATION
DNA-CLONING
ELECTROMAGNETIC RADIATION
EMBRYOS
GENES
HYBRIDIZATION
MAMMALS
MAN
MESSENGER-RNA
MICE
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PLASMIDS
PRIMATES
RADIATIONS
RECOVERY
REPAIR
RNA
RODENTS
SKIN DISEASES
TRANSCRIPTION
ULTRAVIOLET RADIATION
VERTEBRATES
XERODERMA PIGMENTOSUM
XP CELLS