Senescence of nickel-transformed cells by an X chromosome: Possible epigenetic control

Journal Article · Fri Feb 15 00:00:00 EST 1991 · Science (Washington, D.C.); (United States)

DOI:https://doi.org/10.1126/science.1990442· OSTI ID:5596855

Klein, C B; Wang, Xin Wei; Bhamra, R K; Lin, Xinhua; Cohen, M D; Costa, M ^[1]; Conway, K ^[2]; Annab, L; Barrett, J C ^[3]

---

1. New York Univ. Medical Center, NY (United States)
2. Univ. of North Carolina Medical School, Chapel Hill (United States)
3. National Inst. of Environmental Health Sciences, Research Triangle Park, NC (United States)

Transfer of a normal Chinese hamster X chromosome (carried in a mouse A9 donor cell line) to a nickel-transformed Chinese hamster cell line with an Xq chromosome deletion resulted in senescense of these previously immortal cells. At early passages of the A9/CX donor cells, the hamster X chromosome was highly active, inducing senescence in 100% of the colonies obtained after its transfer into the nickel-transformed cells. However, senescence was reduced to 50% when Chinese hamster X chromosomes were transferred from later passage A9 cells. Full senescing activity of the intact hamster X chromosome was restored by treatment of the donor mouse cells with 5-azacytidine, which induced demethylation of DMA. These results suggest that a senescence gene or genes, which may be located on the Chinese hamster X chromosome, can be regulated by DNA methylation, and that escape from senescence and possibly loss of tumor suppressor gene activity can occur by epigenetic mechanisms.

• OSTI ID: 5596855
• Journal Information: Science (Washington, D.C.); (United States), Vol. 251:4995; ISSN 0036-8075
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ONCOGENIC TRANSFORMATIONS
DNA
METHYLATION
NICKEL
CARCINOGENESIS
GENE REGULATION
GENES
HAMSTERS
METABOLISM
X CHROMOSOME
ANIMALS
CELL TRANSFORMATIONS
CHEMICAL REACTIONS
CHROMOSOMES
ELEMENTS
HETEROCHROMOSOMES
MAMMALS
METALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PATHOGENESIS
RODENTS
TRANSITION ELEMENTS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology