Increase of O6-methylguanine-DNA-methyltransferase and N3-methyladenine glycosylase RNA transcripts in rat hepatoma cells treated with DNA-damaging agents
Journal Article
·
· Biochemical and Biophysical Research Communications; (United States)
- U247 INSERM, Institut Gustave Roussy, Villejuif (France)
A variety of DNA-damaging agents increase the O6-methylguanine-DNA-methyltransferase (transferase) and the N3-methyladenine (3-meAde)-DNA-glycosylase activities in a rat hepatoma cell line (H4 cells). Using two cDNA expressing either the rat 3-meAde-DNA-glycosylase or the transferase, the level of mRNA transcripts was measured by hybridization in H4 cells treated with three different inducing agents, gamma-rays, cis-dichlorodiammine platinum II or N-methyl-9-hydroxy ellipticinium. The two mRNA increased 24 hours after the cell treatments but this enhanced transcription was a transient phenomenon, as it was no longer observed after 96 hours. No significant DNA amplification was detectable in the treated cells.
- OSTI ID:
- 5592581
- Journal Information:
- Biochemical and Biophysical Research Communications; (United States), Journal Name: Biochemical and Biophysical Research Communications; (United States) Vol. 176:3; ISSN BBRCA; ISSN 0006-291X
- Country of Publication:
- United States
- Language:
- English
Similar Records
Expression of the O6-methylguanine-DNA methyltransferase gene MGMT in MER+ and MER- human tumor cells
Enhancement of O6-methylguanine-DNA-methyltransferase activity induced by various treatments in mammalian cells
Cloning and characterization of a 3-methyladenine DNA glycosylase cDNA from human cells whose gene maps to chromosome 16
Journal Article
·
Fri Dec 14 23:00:00 EST 1990
· Cancer Research; (USA)
·
OSTI ID:6065082
Enhancement of O6-methylguanine-DNA-methyltransferase activity induced by various treatments in mammalian cells
Journal Article
·
Fri Oct 31 23:00:00 EST 1986
· Cancer Res.; (United States)
·
OSTI ID:7133149
Cloning and characterization of a 3-methyladenine DNA glycosylase cDNA from human cells whose gene maps to chromosome 16
Journal Article
·
Tue Oct 15 00:00:00 EDT 1991
· Proceedings of the National Academy of Sciences of the United States of America; (United States)
·
OSTI ID:5696627
Related Subjects
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTINEOPLASTIC DRUGS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CARBON-GROUP TRANSFERASES
DISEASES
DNA REPAIR
DRUGS
ELECTROMAGNETIC RADIATION
ENZYME ACTIVITY
ENZYMES
GAMMA RADIATION
GENE AMPLIFICATION
GENETIC EFFECTS
GLYCOSYL HYDROLASES
HEPATOMAS
HYBRIDIZATION
HYDROLASES
IONIZING RADIATIONS
MAMMALS
MESSENGER-RNA
METHYL TRANSFERASES
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATIONS
RATS
RECOVERY
REPAIR
RNA
RODENTS
TRANSCRIPTION
TRANSFERASES
TUMOR CELLS
VERTEBRATES
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTINEOPLASTIC DRUGS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CARBON-GROUP TRANSFERASES
DISEASES
DNA REPAIR
DRUGS
ELECTROMAGNETIC RADIATION
ENZYME ACTIVITY
ENZYMES
GAMMA RADIATION
GENE AMPLIFICATION
GENETIC EFFECTS
GLYCOSYL HYDROLASES
HEPATOMAS
HYBRIDIZATION
HYDROLASES
IONIZING RADIATIONS
MAMMALS
MESSENGER-RNA
METHYL TRANSFERASES
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATIONS
RATS
RECOVERY
REPAIR
RNA
RODENTS
TRANSCRIPTION
TRANSFERASES
TUMOR CELLS
VERTEBRATES