Decrease in long-term marrow repopulating capacity seen after transplantation is not the result of irradiation-induced stromal injury

Journal Article · Fri Jan 01 04:00:00 EST 1988 · Exp. Hematol.; (United States)

OSTI ID:5592022

Gardner, R V; Astle, C M; Harrison, D E

Marrow cells from nonirradiated F1-W/Wv mice repopulated slightly less well than cells from lethally irradiated recipients. Therefore, avoiding irradiation of recipients did not improve the relative repopulating ability of their marrow cells. In other experiments, F1-W/Wv mice were transplanted by parabiosis with marrow of WBB6F1-+/+ (F1-+/+) mice, avoiding cellular handling and irradiation. Marrow cells transplanted to F1-W/Wv mice by this procedure demonstrated slightly better repopulating ability than did marrow cells transplanted by injection. However, they performed no better than those transplanted by parabiosis to irradiated F1-+/+ recipients. Significant impairment of stromal function after irradiation was not indicated. Apparently, stem cell damage caused by transplantation may have greater importance in causing loss of stem cell replicative potential than effects of irradiation-induced stromal injury.

• Research Organization: Univ. of Florida College of Medicine, Gainesville
• OSTI ID: 5592022
• Journal Information: Exp. Hematol.; (United States), Journal Name: Exp. Hematol.; (United States) Vol. 16:1; ISSN EXHMA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BONE MARROW CELLS
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
INJURIES
IRRADIATION
LETHAL IRRADIATION
MAMMALS
MICE
MOSAICISM
PARABIOSIS
RADIATION EFFECTS
RADIATION INJURIES
RODENTS
SOMATIC CELLS
STEM CELLS
TRANSPLANTS
VERTEBRATES