Response of fibrosarcoma cell subpopulations to small doses of radiation delivered in situ
Journal Article
·
· Int. J. Radiat. Oncol., Biol. Phys.; (United States)
The survival of cells from 2 tumor subpopulations after gamma-ray doses ranging from 1 to 19 Gy was determined using a lung colony assay. Methylcholanthrene-induced fibrosarcomas grown in the hind legs of C3H/Kam pathogen-free mice were irradiated in situ when the tumors were 8-10 mm in diameter. Single cell suspensions prepared from excised tumors were separated on a linear density gradient, and the clonogenicity of predominantly oxic Band 2 and predominantly hypoxic Band 4 cells was measured. The surviving fraction of cells after doses of 1, 2, and 3 Gy was estimated from that measured after total doses of 5 Gy, 10 Gy, and 15 Gy, under the assumption of equal effect per fraction. Very little curvature was evident in the survival curves of Band 2 and Band 4 cells. The initial segment of the survival curve of the predominantly oxic Band 2 cells was steeper than that of the predominantly hypoxic Band 4 cells; both remained linear over a large range, to doses in excess of 3 Gy. These results imply that these tumor subpopulations will be insensitive, in their response to multifractionated regimens, to changes in size of dose per fraction in the range 0 to 3 Gy, a trait shared by two acutely responding normal tissues (murine testis and jejunum).
- Research Organization:
- M.D. Anderson Hospital and Tumor Inst., Houston, TX
- OSTI ID:
- 5586379
- Journal Information:
- Int. J. Radiat. Oncol., Biol. Phys.; (United States), Journal Name: Int. J. Radiat. Oncol., Biol. Phys.; (United States) Vol. 9:2; ISSN IOBPD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
COLONY FORMATION
DISEASES
ELECTROMAGNETIC RADIATION
FIBROSARCOMAS
FRACTIONATED IRRADIATION
GAMMA RADIATION
IN VIVO
IONIZING RADIATIONS
IRRADIATION
LUNGS
NEOPLASMS
ORGANS
POPULATION DYNAMICS
RADIATION EFFECTS
RADIATIONS
RESPIRATORY SYSTEM
SARCOMAS
SURVIVAL CURVES
TUMOR CELLS
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
COLONY FORMATION
DISEASES
ELECTROMAGNETIC RADIATION
FIBROSARCOMAS
FRACTIONATED IRRADIATION
GAMMA RADIATION
IN VIVO
IONIZING RADIATIONS
IRRADIATION
LUNGS
NEOPLASMS
ORGANS
POPULATION DYNAMICS
RADIATION EFFECTS
RADIATIONS
RESPIRATORY SYSTEM
SARCOMAS
SURVIVAL CURVES
TUMOR CELLS