Effects of intraoperative electron irradiation in the dog on cell turnover in intact and surgically-anastomosed aorta and intestine

Journal Article · Fri Apr 01 04:00:00 EST 1983 · Int. J. Radiat. Oncol., Biol. Phys.; (United States)

DOI:https://doi.org/10.1016/0360-3016(83)90071-8· OSTI ID:5585691

Sindelar, W F; Morrow, B M; Travis, E L; Tepper, J; Merkel, A B; Kranda, K; Terrill, R

Adults dogs were subjected to laparotomy and intraoperative electron irradiation after division and reanastomosis of aorta or after construction of a blind loop of small intestine having a transverse suture line and an end-to-side anastomosis. Dogs received intraoperative irradiation of both intact and anastomosed aorta or intestine in doses of 0, 2000, 3000, or 4500 rad. Animals were sacrificed at seven days or three months following treatment. At 24 hours prior to sacrifice, dogs received 5 mCi tritiated thymidine intravenously. Irradiated and non-irradiated segments of aorta and small intestine, including intact and anastomotic regions, were analyzed for tritiated thymidine incorporation and were subjected to autoradiography. Incorporation studies showed diminution in tritiated thymidine uptake by irradiated portions of aorta and small intestine, in both intact and anastomotic regions. Autoradiograms revealed that irradiated areas of intact or anastomotic aorta or intestine had diminished labeling of stromal cells, suggesting a lowered cell proliferative capacity of irradiated tissue compared to non-irradiated portions. Inflammatory cells showed similar labeling indices in irradiated and non-irradiated tissues, both intact and surgically-manipulated, suggesting that irradiation does not significantly affect a subsequent local inflammatory response. Radiation-induced decreases in tritiated thymidine incoporation in irradiated aorta and small intestine were generally more marked at seven days than at three months following irradiation, suggesting that radiation-induced depression of cell turnover rates decreases with time.

• Research Organization: National Institutes of Health, Bethesda, MD
• OSTI ID: 5585691
• Journal Information: Int. J. Radiat. Oncol., Biol. Phys.; (United States), Journal Name: Int. J. Radiat. Oncol., Biol. Phys.; (United States) Vol. 9:4; ISSN IOBPD
• Country of Publication: United States
• Language: English

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550603 -- Medicine-- External Radiation in Therapy-- (1980-)
560152* -- Radiation Effects on Animals-- Animals
62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AORTA
ARTERIES
AUTORADIOGRAPHY
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BLOOD VESSELS
BODY
CARDIOVASCULAR SYSTEM
CELL PROLIFERATION
DIGESTIVE SYSTEM
ELECTRONS
ELEMENTARY PARTICLES
FERMIONS
GASTROINTESTINAL TRACT
HETEROCYCLIC COMPOUNDS
INTESTINES
LABELLED COMPOUNDS
LEPTONS
MEDICINE
NUCLEAR MEDICINE
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
RADIATION EFFECTS
RADIOLOGY
RADIOTHERAPY
RIBOSIDES
SIDE EFFECTS
SMALL INTESTINE
THERAPY
THYMIDINE
TRITIUM COMPOUNDS