Thromboxane A sub 2 receptors are influenced by cell density in cultured rat aortic smooth muscle cells

Journal Article · Tue Jan 01 00:00:00 EST 1991 · Life Sciences; (USA)

DOI:https://doi.org/10.1016/0024-3205(91)90372-I· OSTI ID:5584346

Masuda, Atsushi; Halushka, P V ^[1]

---

1. Medical Univ. of South Carolina, Charleston (USA)

The influence of cell density on the binding characteristics of thromboxane A{sub 2}/prostaglandin H{sub 2} (TXA{sub 2}/PGH{sub 2}) receptors in rat aortic vascular smooth muscle cells in culture were determined using (1S- (1{alpha}, 2{beta} (5Z), 3a (1E, 3R*), 4{alpha})) - (3- (3-hydroxy-4- (4{prime}-iodophenoxy)-1-butyenyl)-7-oxabicyclo-(2.2.1)heptan-2yl)-5-heptenoic acid ({sup 125}I-BOP). The B{sub max} for {sup 125}I-BOP was 5,430 {plus minus} 139 sites/cell (26.9 {plus minus} 5.7 fmoles/mg protein) for cells cultured in 1% fetal calf serum and 2,809 {plus minus} 830 sites/cell (13.1 {plus minus} 2.2 fmoles/mg protein) for cells cultured in 10% fetal calf serum. Cells were allowed to grow to varying densities and then harvested for assay. There was a negative correlation between the B{sub max} and the cell density per flask. The K{sub d} for I-BOP did not significantly vary in any of the studies. The results demonstrate that cell density plays an important role in influencing the expression of vascular TXA{sub 2}/PGH{sub 2} receptors.

• OSTI ID: 5584346
• Journal Information: Life Sciences; (USA), Vol. 48:25; ISSN 0024-3205
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
DENSITY
PROSTAGLANDINS
RECEPTORS
BIOCHEMICAL REACTION KINETICS
AORTA
CELL CULTURES
IODINE 125
MUSCLES
RATS
TRACER TECHNIQUES
ANIMALS
ARTERIES
BETA DECAY RADIOISOTOPES
BLOOD VESSELS
BODY
CARDIOVASCULAR SYSTEM
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MEMBRANE PROTEINS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHYSICAL PROPERTIES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RODENTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques