Somatostatin binding to dissociated cells from rat cerebral cortex
- Universidad de Alcala, Madrid (Spain)
A method has been developed for the study of somatostatin (SS) binding to dissociated cells from rat cerebral cortex. Binding of {sup 125}I (Tyr11)SS to cells obtained by mechanical dissociation of rat cerebral cortex was dependent on time and temperature, saturable, reversible and highly specific. Under conditions of equilibrium, i.e., 60 min at 25 degrees C, native SS inhibited tracer binding in a dose-dependent manner. The Scatchard analysis of binding data was linear and yielded a dissociation constant of 0.60 +/- 0.08 nM with a maximal binding capacity of 160 +/- 16 fmol/mg protein. The binding of {sup 125}I (Tyr11)SS was specific as shown in experiments on tracer displacement by the native peptides, SS analogues, and unrelated peptides.
- OSTI ID:
- 5583654
- Journal Information:
- Peptides (Fayetteville, New York); (USA), Vol. 11:6; ISSN 0196-9781
- Country of Publication:
- United States
- Language:
- English
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SOMATOSTATIN
BIOCHEMICAL REACTION KINETICS
CEREBRAL CORTEX
DOSE-RESPONSE RELATIONSHIPS
IN VITRO
IODINE 125
PEPTIDES
RATS
TEMPERATURE DEPENDENCE
TIME DEPENDENCE
TRACER TECHNIQUES
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
CEREBRUM
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
NERVOUS SYSTEM
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RODENTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques