Screening for. beta. -glucosidase mutants of Trichoderma reesei with resistance to end-product inhibition

Journal Article · Tue Jan 01 00:00:00 EST 1980 · Dev. Ind. Microbiol.; (United States)

OSTI ID:5579786

Cuskey, S M; Schamhart, D H.J.; Chase, T Jr; Montenecourt, B S; Eveleigh, D E

A simple technique, using an esculin-ferric salt visualization system, for selective isolation of mutants of Trichoderma reesei was employed. The ..beta..-glucosidase crude enzyme complexes of the 17 mutants isolated from some 66,000 colonies screened were characterized. Type of inhibition (competitive, noncompetitive) by glucose, and kinetic parameters K/sub m/ (mM pNPG), V/sub max/ (units/mg extracellular protein), and K/sub i/ slopes (mM glucose) were determined for the mutants using p-nitrophenyl ..beta..-D-glucoside (pNPG) as substrate. All the isolates were inhibited competitively by glucose, but certain of them were less sensitive than parent and wild-type to inhibition by glucose. 5 figures, 1 table.

• Research Organization: Rutgers-The State Univ. of New Jersey, New Brunswick
• OSTI ID: 5579786
• Journal Information: Dev. Ind. Microbiol.; (United States), Vol. 21
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
09 BIOMASS FUELS
ENZYMATIC HYDROLYSIS
INHIBITION
TRICHODERMA VIRIDE
GLUCOSIDASE
MUTANTS
CELLULOSE
GLUCOSE
SCREENING
ALDEHYDES
CARBOHYDRATES
CHEMICAL REACTIONS
DECOMPOSITION
ENZYMES
FUNGI
GLYCOSYL HYDROLASES
HEXOSES
HYDROLASES
HYDROLYSIS
LYSIS
MONOSACCHARIDES
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
SACCHARIDES
SOLVOLYSIS
TRICHODERMA
550700* - Microbiology
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