Sulfur uptake by type I collagen from methyl mercaptan/dimethyl disulfide air mixtures
Type I acid-soluble collagen, suspended in 0.02 M Tris/0.13 M NaCl buffer (pH 7.4), was exposed to air atmosphere admixed with 10.7 X 10(-9) moles of (35S)-labeled dimethyl disulphide/methyl mercaptan mixture in the ratio of 99.82% (CH3S)2/0.18% CH3SH. Gas chromatographic analyses of head-space following one and four days of incubation indicated that all of the CH3SH was absorbed from the head-space by the collagen-containing liquid phase, while the (CH3S)2 concentration of the head-space remained essentially unchanged. Of the total (35S)-activity initially present in the head-space, only 1.0 the total (35S)-activity initially present in the head-space, only 1.0 and 1.86% was absorbed by the collagen-containing liquid phase within one and four days, respectively. Paper chromatographic separation of the reaction mixture showed that only 0.42 and 0.60% of the radioactivity in the head-space was associated with collagen after one and four days of reaction. In comparable (35S)-H2S systems devoid of (CH3S)2, all of the H2S was absorbed within 48 hours of incubation with 18.04% (1 day) and 18.28% (4 days) of the available (35S)-H2S complexed with collagen. The results provide evidence that the reaction of collagen with H2S and CH3SH/(CH3S)2 mixture proceeded via the H2S and CH3SH thiol groups.
- Research Organization:
- Univ. of British Columbia, Vancouver
- OSTI ID:
- 5575352
- Journal Information:
- J. Dent. Res.; (United States), Vol. 12
- Country of Publication:
- United States
- Language:
- English
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COLLAGEN
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ADSORPTION
AIR
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HYDROGEN SULFIDES
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THIOLS
TRACER TECHNIQUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
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NUCLEI
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ORGANIC SULFUR COMPOUNDS
PROTEINS
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RODENTS
SCLEROPROTEINS
SORPTION
SULFIDES
SULFUR COMPOUNDS
SULFUR ISOTOPES
SURFACE PROPERTIES
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)