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Model for validation of radioimmunoassay kit reagents: measurement of follitropin and lutropin in blood and urine

Journal Article · · Clin. Chem. (Winston-Salem, N.C.); (United States)
OSTI ID:5574575
; ; ;
We measured lutropin and follitropin in blood and urine with radioimmunoassay kits from Diagnostic Products Corporation and compared the results with those obtained by use of re agents from the National Institutes of health (NIH) and the World Health Organization (WHO). The urine standard (second IRP-HMG) from WHO, the blood standard (LER-907) from NIH, and the commercial standards all effected similar displacement of trace material when the commercial gonadotropin kit reagents were used. Highly significant correlations were achieved for these hormones in blood or urine on comparing commercial and NIH/WHO reagents. Serial dilutions of urine samples produced similar relative potencies with the commercial reagents. Conversion factors are presented to relate results for LER-907, second IRP, or commercial standards. Commercially available reagents can provide a practical and reliable means of gonadotropin radioimmunoassay in blood or urine.
Research Organization:
Pennsylvania State Univ., Hershey, PA
OSTI ID:
5574575
Journal Information:
Clin. Chem. (Winston-Salem, N.C.); (United States), Journal Name: Clin. Chem. (Winston-Salem, N.C.); (United States) Vol. 27:11; ISSN CLCHA
Country of Publication:
United States
Language:
English

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Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BLOOD
BODY FLUIDS
COMPARATIVE EVALUATIONS
DATA
EVALUATION
EXPERIMENTAL DATA
GONADOTROPINS
HORMONES
INFORMATION
ISOTOPE APPLICATIONS
MATERIALS
NUMERICAL DATA
PEPTIDE HORMONES
PITUITARY HORMONES
RADIOASSAY
RADIOIMMUNOASSAY
REAGENTS
TRACER TECHNIQUES
URINE
WASTES