Use of hydroxyurea in the measurement of DNA repair by the BND cellulose method
Journal Article
·
· Environ. Mutagen.; (United States)
Hydroxyurea inhibition is a convenient method of suppressing replicative DNA synthesis for DNA excision-repair measurement by the BND cellulose technique. Nonetheless, hydroxyurea can introduce artefacts by direct reaction with repair-inducing compounds and by long-term inhibition of the overall repair process. A simple technique of overcoming these problems is described. Cells are reacted with repair-inducing compounds in the absence of hydroxyurea, the cells are washed free of inducer, hydroxyurea is added to 2 mM, and after a short period to establish replication inhibition, /sup 3/H dThd is added and repair measured over a one-hour incubation period.
- Research Organization:
- Univ. of Chicago, IL
- OSTI ID:
- 5569902
- Journal Information:
- Environ. Mutagen.; (United States), Journal Name: Environ. Mutagen.; (United States) Vol. 2; ISSN ENMUD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201 -- Biochemistry-- Tracer Techniques
550301 -- Cytology-- Tracer Techniques
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATING AGENTS
AMIDES
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CARBOHYDRATES
CELLULOSE
DNA
DNA REPLICATION
ESTERS
HYDROXY COMPOUNDS
HYDROXYUREA
INHIBITION
LABELLED COMPOUNDS
METHYL METHANESULFONATE
MUTAGENS
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
POLYSACCHARIDES
RECOVERY
REPAIR
SACCHARIDES
SULFONIC ACID ESTERS
TRITIUM COMPOUNDS
XP CELLS
550301 -- Cytology-- Tracer Techniques
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATING AGENTS
AMIDES
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CARBOHYDRATES
CELLULOSE
DNA
DNA REPLICATION
ESTERS
HYDROXY COMPOUNDS
HYDROXYUREA
INHIBITION
LABELLED COMPOUNDS
METHYL METHANESULFONATE
MUTAGENS
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
POLYSACCHARIDES
RECOVERY
REPAIR
SACCHARIDES
SULFONIC ACID ESTERS
TRITIUM COMPOUNDS
XP CELLS