Elimination of quenching effects in luminescence spectrometry by phase resolution

Journal Article · Tue Jul 01 04:00:00 EDT 1986 · Anal. Chem.; (United States)

DOI:https://doi.org/10.1021/ac00121a026· OSTI ID:5569820

Demas, J N; Jones, W M; Keller, R A

We describe two methods of reducing quenching effects in analytical luminescence measurements. These methods are a form of time resolution based on the amplitudes and the phase shifts of modulated signals. In method 1 the signal is modulated at a high frequency where we show that the signal is essentially independent of sample quenching. Method 2 simultaneously measures the modulated amplitude and the phase shift between the emission and the excitation; using these data, we correct the modulated amplitude to the unquenched value. Method 1 is not as accurate as method 2 but lends itself to use with automated instruments where the sample composition may change and rapid real-time measurements are desired. Method 2 is more accurate but requires more calculations and careful control of experimental conditions. Experimental results are presented on the reduction of errors in the quenching of uranyl by chloride. Our methods are compared with other approaches for eliminating quenching errors.

• Research Organization: Los Alamos National Lab., NM
• OSTI ID: 5569820
• Journal Information: Anal. Chem.; (United States), Journal Name: Anal. Chem.; (United States) Vol. 58:8; ISSN ANCHA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400104* -- Spectral Procedures-- (-1987)
ACCURACY
ACTINIDE COMPOUNDS
BIOLOGICAL MATERIALS
CALCULATION METHODS
CHEMICAL ANALYSIS
CHLORIDES
CHLORINE COMPOUNDS
DE-EXCITATION
EMISSION SPECTROSCOPY
ENERGY LEVELS
ENERGY TRANSFER
ENERGY-LEVEL TRANSITIONS
ENVIRONMENTAL MATERIALS
ERRORS
EXCITED STATES
FREQUENCY MODULATION
HALIDES
HALOGEN COMPOUNDS
LUMINESCENCE
MATERIALS
MODULATION
QUANTITATIVE CHEMICAL ANALYSIS
RADIATIONLESS DECAY
RESOLUTION
SPECTROSCOPY
URANIUM COMPOUNDS
URANYL COMPOUNDS