Physical mapping of a 330 X 10(3)-base-pair region of the Myxococcus xanthus chromosome that is preferentially labeled during spore germination

Journal Article · Mon Apr 01 04:00:00 EST 1985 · J. Bacteriol.; (United States)

OSTI ID:5569308

Komano, T; Inouye, S; Inouye, M

Myxococcus xanthus was pulse-labeled with (/sup 3/H)thymidine immediately after germination of dimethyl sulfoxide-induced spores. The restriction enzyme digests of the total chromosomal DNA from the pulse- labeled cells were analyzed by one-dimensional as well as two- dimensional agarose gel electrophoresis. Four PstI fragments preferentially labeled at a very early stage of germination were cloned into the unique PstI site of pBR322. By using these clones as probes, a restriction enzyme map was established covering approximately 6% of the total M. xanthus genome (330 X 10(3) base pairs). The distribution of the specific activities of the restriction fragments pulse-labeled after germination suggests a bidirectional mode of DNA replication from a fixed origin.

• Research Organization: Tokyo Metropolitan Univ., Japan
• OSTI ID: 5569308
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 162:1; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AZINES
BACTERIA
BACTERIAL SPORES
BIOLOGY
CHROMOSOMES
CLONING
DNA
DNA REPLICATION
ELECTROPHORESIS
GENETIC MAPPING
GENETICS
GERMINATION
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAPPING
MICROORGANISMS
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RIBOSIDES
SPORES
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS