Regulation of 2', 5'-oligoadenylate synthetase gene expression by interferons and platelet-derived growth factor

Journal Article · Wed Mar 01 04:00:00 EST 1989 · Molecular and Cellular Biology; (USA)

DOI:https://doi.org/10.1128/MCB.9.3.1060· OSTI ID:5566226

Garcia-Blanco, M A ^[1]; Lengyel, P ^[2]; Morrison, E; BrownLee, C; Stiles, C D ^[3]; Williams, B R.G. ^[4]

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1. Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA (US)
2. Yale Univ., New Haven, CT (USA). Dept. of Molecular Biophysics and Biochemistry
3. Harvard Medical School, Boston, MA (USA)
4. Toronto Univ., ON (Canada)

In murine BALB/c 3T3 cell cultures, either beta interferon or platelet-derived growth factor (PDGF) enhanced expression of the 2', 5-oligoadenylate synthetase mRNA and protein. The time course of induction in response to beta inteferon was similar to that in response to PDGF. Of several growth factors known to be present in clotted blood serum (i.e., epidermal growth factor, transforming growth factor beta, and PDGF), only PDGF enhanced expression of 2', 5-oligoadenylate synthetase. The linkage of an interferon response element-containing segment from the 5'-flanking region of a human or murine 2'-5'-oligoadenylate synthetase gene made a heterologous gene responsive to interferon. The expression of such a gene construct in transfected cells was also induced by PDGF. Induction by PDGF was inhibited by mono- or polyclonal antibodies to murine interferon, which suggested that induction by PDGF requires interferon. Both PDGF and interferon induced nuclear factors that bound to this interferon response element-containing segment in vitro.

• OSTI ID: 5566226
• Journal Information: Molecular and Cellular Biology; (USA), Journal Name: Molecular and Cellular Biology; (USA) Vol. 9:3; ISSN MCEBD; ISSN 0270-7306
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOCHEMISTRY
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
CELL CULTURES
CHEMISTRY
ENZYMES
FUNCTIONS
GENE AMPLIFICATION
GENE REGULATION
GENETIC MAPPING
GROWTH FACTORS
IN VITRO
INTERFERON
LIGASES
LYMPHOKINES
MAMMALS
MAPPING
MATERIALS
MITOGENS
ORGANIC COMPOUNDS
PROTEINS
RATS
RODENTS
VERTEBRATES