Glucocorticoid inhibition of cellular proliferation in rat hepatoma cell lines
Glucocorticoids were shown to inhibit the growth rate of Fu5 rat hepatoma cells cultured in the presence or absence of serum and thus, induced a more stringent dependence on serum for growth in this cell line. Fu5 cells, made quiescent at low cell density by continuous exposure to glucocorticoid in the absence of serum, were induced with serum and insulin, which subsequently caused a rapid reinitiation of cellular proliferation. Analysis of total RNA isolated from hormone treated Fu5 cells undergoing serum/insulin induction of DNA synthesis revealed a sequential expression of cellular proto-oncogene products in the absence of any immediate changes in intracellular Ca{sup ++} levels. Introduction of functional glucocorticoid receptor genes into both classes of dexamethasone resistant variants restored glucocorticoid responsiveness and suppression of cell growth. The BDS1 rat hepatoma cell line, an Fu5 derived subclone hypersensitive to the antiprofliferation effects of glucocorticoid, was observed to externalize a glucocorticoid suppressible mitogen (GSM) activity capable of mimicking EGF and insulin induced stimulation of ({sup 3}H)thymidine incorporation into serum starved, competant Balb/c 3T3 cells.
- Research Organization:
- California Univ., Berkeley, CA (USA)
- OSTI ID:
- 5564198
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
GLUCOCORTICOIDS
BIOLOGICAL EFFECTS
TUMOR CELLS
CELL PROLIFERATION
BLOOD SERUM
DNA REPLICATION
GENES
HEPATOMAS
INHIBITION
INSULIN
RATS
RECEPTORS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ADRENAL HORMONES
ANIMAL CELLS
ANIMALS
AZINES
CORTICOSTEROIDS
DISEASES
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KETONES
MAMMALS
MEMBRANE PROTEINS
NEOPLASMS
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDE HORMONES
PREGNANES
PROTEINS
PYRIMIDINES
RIBOSIDES
RODENTS
STEROIDS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
550901 - Pathology- Tracer Techniques