Partial purification and characterization of a peroxidase from neonatal rat skin
Thesis/Dissertation
·
OSTI ID:5564116
Peroxidase activity was partially purified from neonatal CFN rat skin. The membrane-bound peroxidase activity was extracted with 0.5 M calcium chloride and was monitored spectrophotometrically at 470 nm with 2-methoxyphenol and hydrogen peroxide as substrates. Subcellular distribution studies indicated the specific activity to be highest and comparable in the 800 {times} g and 8000 {times} g pellets, lowest in the 100,000 {times} g pellet, and absent in the 100,000 {times} g supernatant. The peroxidase activity was partially purified by affinity chromatography on concanavaline-A-sepharose 4-B and by gel filtration using Bio-gel P-150. The apparent molecular weight of the native enzyme as determined by Bio-gel P-200 gel filtration was approximately 42,500 {plus minus} 2,300 daltons. Peroxidase activity increased linearly with increases in protein concentration, time, and guaiacol concentration. Activity was inhibited approximately 75% by 0.1 mM potassium cyanide or 0.05 mM sodium azide. Pyrogallol, hydroquinone, p-cresol, catechol, benzidine, 3,3{prime}-dimethoxybenzidine, tetramethylbenzidine, and p-phenylenediamine were active as substrates for rat skin peroxidase. Rat skin peroxidase was also shown to mediate non-extractable binding of ({sup 3}H)-benzo(a)pyrene-7,8-dihydrodiol and ({sup 3}H)-2-aminofluorene to DNA and protein.
- Research Organization:
- Michigan Univ., Ann Arbor, MI (USA)
- OSTI ID:
- 5564116
- Country of Publication:
- United States
- Language:
- English
Similar Records
Spinach thylakoid polyphenol oxidase isolation, activation, and properties of the native chloroplast enzyme
Chemical structure of the adducts formed by the oxidation of benzidine in the presence of phenols
Purification, properties, and distribution of ascorbate peroxidase in legume root nodules
Journal Article
·
Fri May 01 00:00:00 EDT 1981
· Plant Physiol.; (United States)
·
OSTI ID:6503359
Chemical structure of the adducts formed by the oxidation of benzidine in the presence of phenols
Journal Article
·
Thu Dec 31 23:00:00 EST 1981
· Carcinogenesis (N.Y.); (United States)
·
OSTI ID:5984149
Purification, properties, and distribution of ascorbate peroxidase in legume root nodules
Journal Article
·
Wed Dec 31 23:00:00 EST 1986
· Plant Physiology; (USA)
·
OSTI ID:5081109
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
560300 -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
ANIMALS
AROMATICS
AZIDES
BENZIDINE
BENZOPYRENE
BIOLOGICAL EFFECTS
BODY
CHEMICAL COMPOSITION
CHROMATOGRAPHY
CONCANAVALIN
CONDENSED AROMATICS
CRESOLS
CYANIDES
DEVELOPERS
DISTRIBUTION
DNA
ENZYME ACTIVITY
ENZYMES
FLUORENE
FRACTIONATION
HYDROCARBONS
HYDROXY COMPOUNDS
MAMMALS
MOLECULAR WEIGHT
NEONATES
NITROGEN COMPOUNDS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
PEROXIDASES
PHENOLS
POLYPHENOLS
PYROCATECHOL
RATS
RODENTS
SEPARATION PROCESSES
SKIN
SPECTROPHOTOMETRY
SUBCELLULAR DISTRIBUTION
VERTEBRATES
560300 -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
ANIMALS
AROMATICS
AZIDES
BENZIDINE
BENZOPYRENE
BIOLOGICAL EFFECTS
BODY
CHEMICAL COMPOSITION
CHROMATOGRAPHY
CONCANAVALIN
CONDENSED AROMATICS
CRESOLS
CYANIDES
DEVELOPERS
DISTRIBUTION
DNA
ENZYME ACTIVITY
ENZYMES
FLUORENE
FRACTIONATION
HYDROCARBONS
HYDROXY COMPOUNDS
MAMMALS
MOLECULAR WEIGHT
NEONATES
NITROGEN COMPOUNDS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
PEROXIDASES
PHENOLS
POLYPHENOLS
PYROCATECHOL
RATS
RODENTS
SEPARATION PROCESSES
SKIN
SPECTROPHOTOMETRY
SUBCELLULAR DISTRIBUTION
VERTEBRATES