Chick neural retina adhesion and survival molecule is a retinol-binding protein
Journal Article
·
· J. Cell Biol.; (United States)
A 20,000-D protein called purpurin has recently been isolated from the growth-conditioned medium of cultured embryonic chick neural retina cells. Purpurin is a constituent of adherons and promotes cell-adheron adhesion by interacting with a cell surface heparan sulfate proteoglycan. It also prolongs the survival of cultured neural retina cells. This paper shows that purpurin is a secretory protein that has sequence homology with a human protein synthesized in the liver that transports retinol in the blood, the serum retinol-binding protein (RBP). Purpurin binds (/sup 3/H)retinol, and both purpurin and chick serum RBP stimulate the adhesion of neural retina cells, although the serum protein is less active than purpurin. Purpurin and the serum RBP are, however, different molecules, for the serum protein is approx.3.000 D larger than purpurin and has different silver-staining characteristics. Finally, purpurin supports the survival of dissociated ciliary ganglion cells, indicating that RBPs can act as ciliary neurotrophic factors.
- Research Organization:
- Salk Institute for Biological Studies, San Diego, CA
- OSTI ID:
- 5563908
- Journal Information:
- J. Cell Biol.; (United States), Journal Name: J. Cell Biol.; (United States) Vol. 102; ISSN JCLBA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
550301 -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMAL CELLS
ANTICOAGULANTS
CARBOHYDRATES
DRUGS
GLUCOPROTEINS
HEMATOLOGIC AGENTS
HEPARIN
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MEMBRANE PROTEINS
MUCOPOLYSACCHARIDES
NERVE CELLS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
POLYSACCHARIDES
PROTEINS
RECEPTORS
SACCHARIDES
SOMATIC CELLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VITAMIN A
VITAMINS
550301 -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMAL CELLS
ANTICOAGULANTS
CARBOHYDRATES
DRUGS
GLUCOPROTEINS
HEMATOLOGIC AGENTS
HEPARIN
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MEMBRANE PROTEINS
MUCOPOLYSACCHARIDES
NERVE CELLS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
POLYSACCHARIDES
PROTEINS
RECEPTORS
SACCHARIDES
SOMATIC CELLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VITAMIN A
VITAMINS