Proliferation of the intestinal epithelium and of the regenerating liver of rats with epidermal growth factor deficiency

Journal Article · Mon Sep 01 04:00:00 EDT 1986 · Bull. Exp. Biol. Med. (Engl. Transl.); (United States)

DOI:https://doi.org/10.1007/BF00834430· OSTI ID:5563537

Ivashchenko, Yu D; Gut, I T; Osipova, L A; Garmanchuk, L V; Khranovskaya, L N; Bykorez, A I

The presence of specific receptors for epidermal growth factor (EGF) in hepatocytes and enterocytes, changes in their number during the period of postresection regeneration of the liver, and also the inexplicably high concentrations of this powerful growth factor in the saliva determined the main purpose of this investigation, which was to study the effect of EGF deficiency, produced by submandibular sialadenectomy, on proliferation of the intestinal and hepatic epithelium during postresection regeneration of these organs. The experiments were carried out on rats that received an intraperitoneal injection of /sup 3/H-thymidine. The specific activity of /sup 125/I-EGF was 12,000 cpm/ng. The EGF concentration in the rats' blood serum, saliva, and urine was determined by radioimmunoassay. Bound /sup 125/I-EGF was precipitated. Results indicate that EGF is a regulatory factor which modifies proliferation.

• Research Organization: R. E. Kavetskii Institute for Problems in Oncology, Kiev, USSR
• OSTI ID: 5563537
• Journal Information: Bull. Exp. Biol. Med. (Engl. Transl.); (United States), Journal Name: Bull. Exp. Biol. Med. (Engl. Transl.); (United States) Vol. 101:4; ISSN BEXBA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMAL GROWTH
ANIMAL TISSUES
ANIMALS
AZINES
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RECOVERY
BIOLOGICAL REGENERATION
BIOLOGICAL WASTES
BLOOD SERUM
BODY
BODY FLUIDS
CELL PROLIFERATION
CRYPT CELLS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
EPITHELIUM
GASTROINTESTINAL TRACT
GLANDS
GROWTH
HETEROCYCLIC COMPOUNDS
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
INTESTINES
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LABELLING
LIVER
LIVER CELLS
MAMMALS
MATERIALS
MEMBRANE PROTEINS
METABOLIC ACTIVATION
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PEPTIDES
POLYPEPTIDES
PROTEINS
PYRIMIDINES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
RATS
RECEPTORS
RECOVERY
RESPONSE MODIFYING FACTORS
RETENTION
RIBOSIDES
RODENTS
SALIVA
SOMATIC CELLS
THYMIDINE
TISSUES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
URINE
VERTEBRATES
WASTES