Proliferation of the intestinal epithelium and of the regenerating liver of rats with epidermal growth factor deficiency
The presence of specific receptors for epidermal growth factor (EGF) in hepatocytes and enterocytes, changes in their number during the period of postresection regeneration of the liver, and also the inexplicably high concentrations of this powerful growth factor in the saliva determined the main purpose of this investigation, which was to study the effect of EGF deficiency, produced by submandibular sialadenectomy, on proliferation of the intestinal and hepatic epithelium during postresection regeneration of these organs. The experiments were carried out on rats that received an intraperitoneal injection of /sup 3/H-thymidine. The specific activity of /sup 125/I-EGF was 12,000 cpm/ng. The EGF concentration in the rats' blood serum, saliva, and urine was determined by radioimmunoassay. Bound /sup 125/I-EGF was precipitated. Results indicate that EGF is a regulatory factor which modifies proliferation.
- Research Organization:
- R. E. Kavetskii Institute for Problems in Oncology, Kiev, USSR
- OSTI ID:
- 5563537
- Journal Information:
- Bull. Exp. Biol. Med. (Engl. Transl.); (United States), Vol. 101:4; Other Information: Translated from Byulleten' Ehksperimental'noj Biologii i Meditsiny; 101: No. 4, 475-478(Apr 1986)
- Country of Publication:
- United States
- Language:
- English
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CRYPT CELLS
BIOLOGICAL REGENERATION
CELL PROLIFERATION
RADIOIMMUNOASSAY
LIVER CELLS
POLYPEPTIDES
BIOLOGICAL EFFECTS
RECEPTORS
RATS
INTESTINES
LIVER
ANIMAL GROWTH
BLOOD SERUM
EPITHELIUM
IODINE 125
LABELLED COMPOUNDS
LABELLING
METABOLIC ACTIVATION
RESPONSE MODIFYING FACTORS
RETENTION
SALIVA
THYMIDINE
TRITIUM COMPOUNDS
URINE
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AZINES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL RECOVERY
BIOLOGICAL WASTES
BODY
BODY FLUIDS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
GASTROINTESTINAL TRACT
GLANDS
GROWTH
HETEROCYCLIC COMPOUNDS
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MATERIALS
MEMBRANE PROTEINS
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PEPTIDES
PROTEINS
PYRIMIDINES
RADIOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
RECOVERY
RIBOSIDES
RODENTS
SOMATIC CELLS
TISSUES
TRACER TECHNIQUES
VERTEBRATES
WASTES
550301* - Cytology- Tracer Techniques
550501 - Metabolism- Tracer Techniques