Specific deuteration of dichlorobenzoate during reductive dehalogenation by Desulfomonile tiedjei in D sub 2 O

Journal Article · Tue Dec 31 23:00:00 EST 1991 · Applied and Environmental Microbiology; (United States)

OSTI ID:5563313

Griffith, G D; Cole, J R; Quensen, III, J F; Tiedje, J M ^[1]

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1. Michigan State Univ., East Lansing (United States)

Desulfomonile tiedjei DCB-1 is a strict anaerobe capable of reductively dechlorinating meta-chlorobenzoates. To probe the mechanism of this aryl dechlorination, the authors incubated cell suspensions of D. tiedjei in D{sub 2}O and with 2,5-dichlorobenzoate. The deuterium was incorporated into the dechlorination product exclusively at the position of dehalogenation, as shown by gas chromatography-mass spectrometry and proton magnetic resonance analyses. These results favor a model for dechlorination that should not allow proton exchange at other positions, as would be the case if partial ring reduction occurred.

• OSTI ID: 5563313
• Journal Information: Applied and Environmental Microbiology; (United States), Journal Name: Applied and Environmental Microbiology; (United States) Vol. 58:1; ISSN 0099-2240; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
540211* -- Environment
Terrestrial-- Basic Studies-- Radiometric Techniques-- (1990-)
ANAEROBIC CONDITIONS
AROMATICS
BACTERIA
BIODEGRADATION
CHEMICAL REACTIONS
CHLORINATED AROMATIC HYDROCARBONS
DECHLORINATION
DECOMPOSITION
DEHALOGENATION
DEUTERATION
HALOGENATED AROMATIC HYDROCARBONS
HEAVY WATER
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
MICROORGANISMS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
OXYGEN COMPOUNDS
TRACER TECHNIQUES
WATER