Complete sequence of HLA-B27 cDNA identified through the characterization of structural markers unique to the HLA-A, -B, and -C allelic series

Journal Article · Sat Mar 01 00:00:00 EST 1986 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.83.5.1428· OSTI ID:5563011

Szoets, H; Reithmueller, G; Weiss, E; Meo, T

Antigen HLA-B27 is a high-risk genetic factor with respect to a group of rheumatoid disorders, especially ankylosing spondylitis. A cDNA library was constructed from an autozygous B-cell line expressing HLA-B27, HLA-Cw1, and the previously cloned HLA-A2 antigen. Clones detected with an HLA probe were isolated and sorted into homology groups by differential hybridization and restriction maps. Nucleotide sequencing allowed the unambiguous assignment of cDNAs to HLA-A, -B, and -C loci. The HLA-B27 mRNA has the structure features and the codon variability typical of an HLA class I transcript but it specifies two uncommon amino acid replacements: a cysteine in position 67 and a serine in position 131. The latter substitution may have functional consequences, because it occurs in a conserved region and at a position invariably occupied by a species-specific arginine in humans and lysine in mice. The availability of the complete sequence of HLA-B27 and of the partial sequence of HLA-Cw1 allows the recognition of locus-specific sequence markers, particularly, but not exclusively, in the transmembrane and cytoplasmic domains.

• Research Organization: Institut fuer Immunologie der Universitaet Muenchen, West Germany
• OSTI ID: 5563011
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 83:5
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANTIGENS
MOLECULAR BIOLOGY
RECOMBINANT DNA
DNA SEQUENCING
BIOLOGICAL MARKERS
HUMAN POPULATIONS
MAN
MESSENGER-RNA
RHEUMATIC DISEASES
ANIMALS
DISEASES
DNA
MAMMALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
POPULATIONS
PRIMATES
RNA
SKELETAL DISEASES
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550200* - Biochemistry